PMID: 429211Feb 26, 1979Paper

Supercontraction in crayfish muscle: correlation with a peculiar actin localization

Histochemistry
G BenzonanaG Gabbiani

Abstract

Crayfish muscle, like muscles from some other invertebrates, can supercontract. This muscle shortening is characterized by an overlap of thin filaments with crossing of thick filaments through the Z discs. In intact muscle cells, supercontraction does not seem to induce irreversible structural modifications in the tissue. Isolated crayfish myofibrils in the relaxed state cannot be distinguished from vertebrate myofibrils under light microscope, either by phase contrast or by immunofluorescence, with antiactin antibodies, actin being localized in the I bands. However, when isolated crayfish myofibrils are supercontracted, irreversible dammage occurs, most thin filaments being lost. Actin becomes then hardly detectable, being visible, by immunofluorescence, either in the Z discs or evenly distributed in the whole myofibril. During myofibril supercontraction, high amounts of denatured actin, become soluble as shown by SDS-PAGE, by double immunodiffusion, and by DNAse inhibition.

References

Apr 1, 1976·Cell·S E HitchcockU Lindberg
Nov 1, 1977·The Journal of Cell Biology·T WallimannH M Eppenberger
Nov 1, 1977·European Journal of Biochemistry·C W Heizmann, M T Häuptle
Jan 25, 1978·Biochimica Et Biophysica Acta·M MugurumaT Fukazawa
Sep 1, 1976·The Journal of General Physiology·M Kawai, P W Brandt
Jan 1, 1976·The Journal of Cell Biology·J D EtlingerD A Fischman
Jan 1, 1976·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J A CoxG Benzonana
Sep 1, 1973·The Journal of Cell Biology·C Franzini-Armstrong
Sep 15, 1973·Journal of Molecular Biology·J D Saide, W C Ullrick
Jan 1, 1968·Progress in Biophysics and Molecular Biology·S Ebashi, M Endo
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·P CuatrecasasC B Anfinsen
Aug 1, 1965·The Journal of Cell Biology·G HoyleA Selverston
Feb 10, 1964·Biochimica Et Biophysica Acta·U LINBERG

❮ Previous
Next ❯

Citations

Feb 27, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Weilin ZhouJ Usha Raj

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.