PMID: 43456Oct 3, 1979

Superhelical DNA in Streptococcus sanguis: role in recombination in vivo

Molecular & General Genetics : MGG
J L Raina, A W RAVIN

Abstract

Competent Streptococcus sanguis treated with non-lethal doses of coumermycin A1 immediately before or after uptake of radioactive transforming DNA were reduced in their capacity to yield transformants. This treatment did not alter bacterial ability to bind DNA in DNase I-resistant form, nor did it prevent the single-stranded donor DNA-recipient protein complexes formed upon uptake at the surface of the bacteria from translocating to chromosomal sites. Inhibition of transformation by heterospecific DNA was greater than that by homospecific DNA. The reduction in transformant yield was not accompanied by any loss of donor counts incorporated into the recipient chromosome, but rather by a loss of genetic activity of incorporated donor material indicating a failure of genetic integration and degradation of donor DNA as a consequence of coumermycin treatment. The inhibitory effect of coumermycin on transformation was associated with in vivo loss of chromosomal DNA superhelicity, The chromosomal DNA remained intact, however, indicative of inhibition of a gyrase-like enzyme responsible for the maintenance of negative supercoiling of the S. sanguis chromosome. Upon treatment with the drug, a coumermycin-resistant mutant strain showed ne...Continue Reading

Citations

Jan 1, 1978·Annual Review of Biochemistry·C M Radding
Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D E Depew, J C Wang
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M GellertH A Nash
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A SuginoN R Cozzarelli
Oct 20, 1977·Nature·B Alberts, R Sternglanz
Jan 1, 1978·Annual Review of Biochemistry·J J Champoux
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·K J MariansJ Hurwitz
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M GellertJ Tomizawa
Apr 5, 1978·Journal of Molecular Biology·K Drlica, M Snyder
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·N P HigginsN R Cozzarelli
May 25, 1978·Journal of Molecular Biology·K MizuuchiH A Nash
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M GellertJ Tomizawa
Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D E PulleyblankH P Vosberg
Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K Mizuuchi, H A Nash
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·W K HollomanC M Radding
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J D Griffith
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J G Tiraby, M S Fox
Apr 14, 1967·Journal of Molecular Biology·L V Crawford, M J Waring
Nov 14, 1972·Journal of Molecular Biology·A Worcel, E Burgi
Jan 1, 1974·Cold Spring Harbor Symposia on Quantitative Biology·H Delius, A Worcel
Jan 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·O G Stonington, D E Pettijohn
Feb 14, 1971·Journal of Molecular Biology·J C Wang

Related Concepts

Antibiotic Resistance, Microbial
Transformation, Genetic
Deoxyribonuclease I
Bacteriocidal Agents
Complex (molecular entity)
Uptake
Recombination, Genetic
Coumermycin
Transformation, Bacterial
Coumarins

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