Superinfection by PHYVV Alters the Recovery Process in PepGMV-Infected Pepper Plants.

Viruses
Myriam G Rodríguez-GandarillaRafael F Rivera-Bustamante

Abstract

Geminiviruses are important plant pathogens that affect crops around the world. In some geminivirus-host interactions, infected plants show recovery, a phenomenon characterized by symptom disappearance in newly emerging leaves. In pepper-Pepper golden mosaic virus (PepGMV) interaction, the host recovery process involves a silencing mechanism that includes both post-transcriptional (PTGS) and transcriptional (TGS) gene silencing pathways. Under field conditions, PepGMV is frequently found in mixed infections with Pepper huasteco yellow vein virus (PHYVV), another bipartite begomovirus. Mixed infected plants generally show a synergetic phenomenon and do not present recovery. Little is known about the molecular mechanism of this interaction. In the present study, we explored the effect of superinfection by PHYVV on a PepGMV-infected pepper plant showing recovery. Superinfection with PHYVV led to (a) the appearance of severe symptoms, (b) an increase of the levels of PepGMV DNA accumulation, (c) a decrease of the relative methylation levels of PepGMV DNA, and (d) an increase of chromatin activation marks present in viral minichromosomes. Finally, using heterologous expression and silencing suppression reporter systems, we found tha...Continue Reading

References

Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Jan 1, 1995·Archives of Virology·C R Pringle
Sep 1, 1994·Virology·G SunterD M Bisaro
Apr 1, 1997·The Journal of General Virology·G M Bonilla-RamírezR F Rivera-Bustamante
Jun 23, 1998·The Plant Cell·M T RuizD C Baulcombe
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·O VoinnetD C Baulcombe
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 3, 2003·The Plant Cell·Linhui HaoDavid M Bisaro
Feb 21, 2004·The EMBO Journal·Lóránt LakatosJózsef Burgyán
Jul 15, 2005·Journal of Virology·Sharon B SettlageLinda Hanley-Bowdoin
Dec 3, 2005·Nature Reviews. Microbiology·Marilyn J Roossinck
Dec 21, 2005·Virology·David M Bisaro
Jul 4, 2008·Journal of Virology·Priya RajaDavid M Bisaro
Sep 13, 2008·BMC Bioinformatics·Eyal GruntmanRavi Sachidanandam
Oct 24, 2008·Phytopathology·Jimena Carrillo-TrippRafael F Rivera-Bustamante
Jan 19, 2010·Biochimica Et Biophysica Acta·Priya RajaDavid M Bisaro
Mar 10, 2011·Virology Journal·Ilenia Rentería-CanettRafael F Rivera-Bustamante
Jul 15, 2011·Molecular Plant-microbe Interactions : MPMI·Imran AminRob W Briddon
Apr 26, 2013·Molecular Plant Pathology·Vincent N Fondong
Oct 9, 2013·Nature Reviews. Microbiology·Linda Hanley-BowdoinShahid Mansoor
Oct 17, 2013·Nature Reviews. Microbiology·Nathan Pumplin, Olivier Voinnet
May 29, 2014·The Journal of General Virology·Yaqin WangXueping Zhou
Oct 11, 2014·Methods in Molecular Biology·Jung MinChee Hark Harn
Jan 2, 2015·Journal of Virology·Jamie N JackelDavid M Bisaro
Jan 16, 2015·Journal of Virology·Björn KrenzHolger Jeske

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Citations

Sep 2, 2020·Journal of Experimental Botany·Fauzia Zarreen, Supriya Chakraborty
Aug 24, 2021·Applied Microbiology and Biotechnology·Dibyendu GhoshSupriya Chakraborty

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Methods Mentioned

BETA
PCR
immunoprecipitation
reverse transcription PCR
Two-hybrid

Software Mentioned

Kismeth

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