Abstract
Three cross-linkable phospholamban (PLB) mutants of increasing inhibitory strength (N30C-PLB < N27A,N30C,L37A-PLB (PLB3) < N27A,N30C,L37A,V49G-PLB (PLB4)) were used to determine whether PLB decreases the Ca(2+) affinity of SERCA2a by competing for Ca(2+) binding. The functional effects of N30C-PLB, PLB3, and PLB4 on Ca(2+)-ATPase activity and E1 approximately P formation were correlated with their binding interactions with SERCA2a measured by chemical cross-linking. Successively higher Ca(2+) concentrations were required to both activate the enzyme co-expressed with N30C-PLB, PLB3, and PLB4 and to dissociate N30C-PLB, PLB3, and PLB4 from SERCA2a, suggesting competition between PLB and Ca(2+) for binding to SERCA2a. This was confirmed with the Ca(2+) pump mutant, D351A, which is catalytically inactive but retains strong Ca(2+) binding. Increasingly higher Ca(2+) concentrations were also required to dissociate N30C-PLB, PLB3, and PLB4 from D351A, demonstrating directly that PLB antagonizes Ca(2+) binding. Finally, the specific conformation of E2 (Ca(2+)-free state of SERCA2a) that binds PLB was investigated using the Ca(2+)-pump inhibitors thapsigargin and vanadate. Cross-linking assays conducted in the absence of Ca(2+) showed t...Continue Reading
References
Oct 19, 1979·Biochimica Et Biophysica Acta·L R Jones
Apr 15, 1992·The Biochemical Journal·M WictomeJ M East
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K Maruyama, D H MacLennan
Apr 26, 1985·Biochimica Et Biophysica Acta·J P Andersen, J V Møller
Nov 6, 1973·Biochemistry·H Masuda, L de Meis
Nov 10, 1981·Biochemistry·T WatanabeG Inesi
Oct 18, 1993·FEBS Letters·F DeJesusY Dupont
Mar 11, 1997·Biochemistry·R L CorneaD D Thomas
Jun 13, 1997·The Journal of Biological Chemistry·Y KimuraD H MacLennan
Jun 20, 1997·The Journal of Biological Chemistry·J M Autry, L R Jones
Jun 11, 1998·The Journal of Biological Chemistry·Y KimuraD H MacLennan
Oct 28, 1998·Physiological Reviews·H K Simmerman, L R Jones
Apr 9, 1999·Biochemistry·L G ReddyD D Thomas
Aug 28, 1999·The Journal of Biological Chemistry·D B McIntoshJ P Andersen
Feb 29, 2000·Biophysical Journal·J E MahaneyL R Jones
Jul 29, 2000·Biochemistry·Z ZhangC Toyoshima
Aug 19, 2000·Circulation Research·D M Bers
Oct 4, 2000·The Journal of Biological Chemistry·R L CorneaL R Jones
May 1, 2001·The Journal of Biological Chemistry·K HaghighiE G Kranias
Jul 11, 2001·The Journal of Biological Chemistry·M I ForteaF Fernandez-Belda
May 17, 2002·The Journal of Biological Chemistry·Larry R JonesZhenhui Chen
Jan 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Chikashi ToyoshimaDavid H MacLennan
Apr 16, 2003·Biochemistry·Laxma G ReddyDavid D Thomas
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·David H MacLennan, Evangelia G Kranias
Sep 16, 2003·The Journal of Biological Chemistry·Zhenhui ChenLarry R Jones
Mar 2, 2005·Biochemistry·Catharine A TrieberHoward S Young
Mar 24, 2006·The Journal of Biological Chemistry·Zhenhui ChenLarry R Jones
May 20, 2006·The EMBO Journal·Anne-Marie Lund JensenPoul Nissen
Jan 30, 2007·The Journal of Biological Chemistry·Karine MoncoqHoward S Young
Jan 31, 2007·Biochemistry·Jason R WaggonerJames E Mahaney
Jun 6, 2007·The Journal of Biological Chemistry·Zhenhui ChenLarry R Jones
Nov 3, 2007·Circulation Research·Seth L RobiaDavid D Thomas
Nov 13, 2007·The Journal of Biological Chemistry·Giuseppe InesiLeopoldo de Meis
Dec 7, 2007·Biochemistry·Cédric MontignyPhilippe Champeil
Feb 22, 2008·The Journal of Biological Chemistry·Eileen M KellySeth L Robia
Mar 22, 2008·The Journal of Biological Chemistry·Alexandre MarchandMarc le Maire
May 6, 2008·Archives of Biochemistry and Biophysics·Chikashi Toyoshima
Oct 25, 2008·Biochemistry·Cédric MontignyPhilippe Champeil
Aug 28, 2009·Biochemistry·Catharine A TrieberHoward S Young
Dec 2, 2009·The Journal of Biological Chemistry·Zhenhui ChenLarry R Jones
Citations
Mar 5, 2013·Nature·Chikashi ToyoshimaGiuseppe Inesi
Aug 13, 2011·The Journal of Biological Chemistry·Philip BidwellSeth L Robia
Jan 17, 2012·The Journal of Biological Chemistry·Brandy L Akin, Larry R Jones
Jan 24, 2013·The Journal of Biological Chemistry·Sanjaya K SahooMuthu Periasamy
Jul 19, 2012·PloS One·Zhanjia HouSeth L Robia
Nov 28, 2013·Journal of Molecular and Cellular Cardiology·Syevda SirenkoEdward G Lakatta
Sep 10, 2014·Journal of Molecular and Cellular Cardiology·Zhenhui Chen
Jan 7, 2015·Journal of Molecular and Cellular Cardiology·Tanja WittmannJoachim P Schmitt
Jan 9, 2016·Journal of Molecular and Cellular Cardiology·Sana A ShaikhMuthu Periasamy
Sep 5, 2015·The FEBS Journal·Zhenhui Chen
Feb 12, 2014·The Journal of Physical Chemistry. B·Xueting Yu, Gary A Lorigan
Oct 22, 2013·Biophysical Journal·Sandeep PallikkuthSeth L Robia
Jul 7, 2011·The Biochemical Journal·Zhenhui Chen
Apr 12, 2015·Biophysical Journal·L Michel Espinoza-FonsecaDavid D Thomas
Sep 17, 2014·PloS One·Eleri Hughes, David A Middleton
Jul 26, 2018·International Journal of Molecular Sciences·Tatiana M VinogradovaEdward G Lakatta
Sep 3, 2013·The Journal of Biological Chemistry·Brandy L AkinLarry R Jones
Aug 26, 2014·Biological Chemistry·Grant Kemp, Florian Cymer
Jun 20, 2020·The Journal of Biological Chemistry·Rebecca F AlfordSeth L Robia
Sep 13, 2013·The Journal of Biological Chemistry·Irene C MangialavoriJuan Pablo F C Rossi
Nov 17, 2020·Frontiers in Physiology·Zhe WangYun-Fang Gao
May 13, 2021·ELife·Daniel K WeberGianluigi Veglia