Superior osteogenic capacity of human embryonic stem cells adapted to matrix-free growth compared to human mesenchymal stem cells

Tissue Engineering. Part a
Narmin BigdeliCamilla Karlsson

Abstract

Human mesenchymal stem cells (hMSCs) represent a promising source of cells for bone tissue engineering. However, their low frequencies and limited proliferation restrict their clinical utility. An alternative is the use of human embryonic stem cells (hESCs), but labor-intensive expansion with the need for coating support limits their clinical use. We have previously derived a cell line from hESCs denoted matrix-free growth (MFG)-hESC that are independent of coating support for expansion, and we here compare its osteogenic capacity to that of hMSCs. Microarray analysis of hMSCs and MFG-hESCs revealed differential expression of genes involved in ossification. MFG-hESCs have significantly higher expression of secreted phosphoprotein 1 (SPP1) during osteogenic differentiation, whereas the opposite was true for alkaline phosphatase (ALPL), transforming growth factor, beta 1 (TGFB2), runt-related transcription factor 2 (RUNX2), and forkhead box C1 (FOXC1), as well as the activity of the ALPL enzyme, demonstrating that these two cell types differentiate into the osteogenic lineage using different signaling pathways. von Kossa staining, time-of-flight secondary ion mass spectrometry, and measurement of calcium and phosphate in the extr...Continue Reading

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Citations

Jun 27, 2012·Tissue Engineering. Part a·Hagit DomevJoseph Itskovitz-Eldor
Jun 7, 2014·International Journal of Nanomedicine·Giuseppe Maria de PeppoSarunas Petronis
Dec 3, 2014·Current Opinion in Biotechnology·Yelena Ilin, Mary L Kraft
Aug 7, 2012·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Vladimir PetrovicVladisav Stefanovic
Jan 24, 2017·European Journal of Medical Genetics·Paolo FontanaMaria Antonietta Pisanti

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Methods Mentioned

BETA
FACS
PCR

Software Mentioned

GeneChip Operating Software
Genesis
GCOS
STRING
Primer3
BIORETIS
FACS Diva

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