Superior protective and therapeutic effects of IL-12 and IL-18 gene-transduced dendritic neuroblastoma fusion cells on liver metastasis of murine neuroblastoma

The Journal of Immunology : Official Journal of the American Association of Immunologists
Hisae IinumaMichio Kaneko

Abstract

Fusion vaccine of dendritic cells (DCs) and tumor cells has the advantage of inducing an immune response against multiple tumor Ags, including unknown tumor Ags. Using the liver metastasis model of C1300 neuroblastoma cells, we assessed the protective and therapeutic effects of fusion cells transduced with the IL-12 gene and/or the IL-18 gene. Improving the fusion method by combining polyethylene glycol and electroporation increased loading efficiency. In the A/J mice vaccinated with fusion cells modified with the LacZ gene (fusion/LacZ), IFN-gamma production and CTL activity increased significantly compared with that of DCs/LacZ, C1300/LacZ, or a mixture of the two (mixture/LacZ). With the transduction of IL-12 and IL-18 genes into the fusion cells (fusion/IL-12/IL-18), the level of IFN-gamma increased more than five times that of other fusion groups. In addition, NK cell activity and CTL activity increased significantly compared with that of mixture/LacZ, fusion/LacZ, DC/LacZ, or C1300/LacZ. In the protective and therapeutic studies of fusion cell vaccine, mice vaccinated with fusion/LacZ, fusion/IL-12, fusion/IL-18, or fusion/IL-12/IL-18 showed a significant decrease in liver metastasis and a significant increase in survival...Continue Reading

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Citations

Feb 26, 2010·Clinical & Developmental Immunology·Shigeo KoidoHisao Tajiri
Jan 1, 2009·Immunotherapy·Shigeo KoidoHisao Tajiri
May 5, 2011·Journal of Biomedicine & Biotechnology·Shigeo KoidoHisao Tajiri
Mar 26, 2015·Immunotherapy·Michela CroceSilvano Ferrini
Sep 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shigeo KoidoHisao Tajiri
Aug 3, 2007·Expert Review of Vaccines·Owen ProudfootGeoffrey A Pietersz
Jul 15, 2011·Immunotherapy·Vito PistoiaLizzia Raffaghello
Apr 27, 2011·Cancer Immunology, Immunotherapy : CII·Masato KatoKingo Chida
Apr 25, 2014·Expert Review of Vaccines·Laetitia CiccheleroNiek N Sanders
Aug 3, 2012·Immunotherapy·Arpit BhargavaPradyumna Kumar Mishra
Dec 9, 2014·Human Vaccines & Immunotherapeutics·Maria TagliamonteLuigi Buonaguro
May 31, 2016·International Journal of Molecular Sciences·Shigeo Koido
Apr 10, 2010·Journal of Biomedicine & Biotechnology·Shigeo KoidoHisao Tajiri
Nov 5, 2010·Clinical & Developmental Immunology·Shigeo KoidoHisao Tajiri
Aug 7, 2012·The Journal of Biological Chemistry·Cristhian J IldefonsoRichard L Hurwitz

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