Superior sensitivity of novel molecular imaging probe: simultaneously targeting two types of endothelial injury markers.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Dawei SunAli Hafezi-Moghadam

Abstract

The need remains great for early diagnosis of diseases. The special structure of the eye provides a unique opportunity for noninvasive light-based imaging of fundus vasculature. To detect endothelial injury at the early and reversible stage of adhesion molecule up-regulation, we generated novel imaging agents that target two distinct types of endothelial molecules, a mediator of rolling, P-selectin, and one that mediates firm adhesion, ICAM-1. Interactions of these double-conjugated fluorescent microspheres (MSs) in retinal or choroidal microvasculature were visualized in live animals by scanning laser ophthalmoscopy. The new imaging agents showed significantly higher sensitivity for detection of endothelial injury than singly conjugated MSs (rPSGL-1- or alpha-ICAM-1-conjugated), both in terms of rolling (P<0.01) and firm adhesion (P<0.01). The rolling flux of alpha-ICAM-1-conjugated MSs did not differ in EIU animals, whereas double-conjugated MSs showed significantly higher rolling flux (P<0.01), revealing that ICAM-1 in vivo supports rolling, once MS interaction with the endothelium is initiated. Double-conjugated MSs specifically detected firmly adhering leukocytes (P<0.01), allowing in vivo quantification of immune response...Continue Reading

References

Aug 7, 1980·Nature·J T RosenbaumP R Egbert
Jan 1, 1995·Retina·P M BischoffP Speiser
Jun 25, 1998·Leukemia & Lymphoma·K L Moore
Mar 17, 1999·The Journal of Experimental Medicine·A Hafezi-Moghadam, K Ley
Apr 3, 2001·The Journal of Experimental Medicine·A Hafezi-MoghadamK Ley
Jul 20, 2004·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·O M DurraniP I Murray
Oct 18, 2005·Progress in Retinal and Eye Research·Yuya Hirata, Hirokazu Nishiwaki
May 26, 2006·Investigative Ophthalmology & Visual Science·Xue-Hai JinShigeaki Ohno
Jan 5, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Hafezi-MoghadamE S Gragoudas
Jan 25, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shinsuke MiyaharaAli Hafezi-Moghadam

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Citations

Jan 25, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rebecca C GarlandAli Hafezi-Moghadam
Jul 21, 2012·Investigative Radiology·Thierry BettingerFrançois Tranquart
Mar 1, 2012·Journal of Ophthalmology·Fang XieDawei Sun
Feb 23, 2013·Diabetes Care·M Kamran IkramUNKNOWN NIH/JDRF Workshop on Retinal Biomarker for Diabetes Group
Jul 11, 2013·Journal of Ophthalmology·Wenting LuoDawei Sun
Jun 17, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Iason PapademetriouSilvia Muro
May 3, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Andreas H JacobsUNKNOWN INMiND consortium
Oct 6, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Vladimir V ShuvaevVladimir R Muzykantov
Jun 29, 2010·The American Journal of Pathology·Fang XieAli Hafezi-Moghadam
Mar 26, 2011·The American Journal of Pathology·Shintaro NakaoAli Hafezi-Moghadam
Jun 7, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dawei SunAli Hafezi-Moghadam
Sep 15, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shintaro NakaoAli Hafezi-Moghadam
Jul 3, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Aliaa BarakatAli Hafezi-Moghadam
Jan 10, 2017·Bioengineering & Translational Medicine·Catherine A FromenOmolola Eniola-Adefeso
Sep 11, 2019·Progress in Retinal and Eye Research·Yusuke MurakamiKoh-Hei Sonoda
Jun 25, 2020·Advanced Drug Delivery Reviews·Patrick M GlassmanVladimir R Muzykantov

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