PMID: 6987655Jan 1, 1980Paper

"Superkiller" mutations suppress chromosomal mutations affecting double-stranded RNA killer plasmid replication in saccharomyces cerevisiae

Proceedings of the National Academy of Sciences of the United States of America
A Toh-E, R B Wickner

Abstract

Saccharomyces cerevisiae strains carrying a 1.5 x 10(6)-dalton double-stranded RNA genome in virus-like particles (killer plasmid) secrete a protein toxin that kills strains not carrying this plasmid. At least 28 chromosomal genes (mak genes) are required to maintain or replicate this plasmid. Recessive mutations in any of four other chromosomal genes (ski for superkiller) result in enhanced toxin production. We report that many ski- mak- double mutants are able to maintain the killer plasmid, indicating that the SKI products have an effect on plasmid replication. The ski1-1 mutation suppresses (bypasses) all mak mutations tested except mak16-1. A variant killer plasmid is described that confers the superkiller phenotype and, like chromosomal ski mutations, makes several mak genes dispensable for plasmid replication.

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H M Fried, G R Fink
Jan 1, 1978·Antonie van Leeuwenhoek·T W Young, M Yagiu
Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J Conde, G R Fink
Jan 1, 1973·Annual Review of Genetics·A J Clark
Jan 19, 1972·Nature: New Biology·H Bussey
Oct 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·G R Fink, C A Styles
Feb 1, 1969·Genetical Research·J M Somers, E A Bevan

❮ Previous
Next ❯

Citations

Jan 1, 1982·Molecular & General Genetics : MGG·J Polaina, J Conde
Jan 1, 1986·Folia Microbiologica·O Bendová
Aug 8, 2002·FEMS Microbiology Reviews·Manfred J Schmitt, Frank Breinig
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T C ArmstrongJ L Patterson
Oct 12, 2000·Molecular and Cellular Biology·A van HoofR Parker
Jan 1, 1996·Annual Review of Genetics·R B Wickner
Nov 1, 1993·Trends in Microbiology·R B Wickner
Apr 30, 1981·Biochemical and Biophysical Research Communications·B L Brizzard, S R de Kloet
Oct 7, 2016·PLoS Pathogens·Paul A RowleySara L Sawyer
Jan 1, 1991·Critical Reviews in Microbiology·L PolonelliG Morace
Jan 1, 1987·Critical Reviews in Biotechnology·R E Subden
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·J E McCarthy
Mar 1, 1996·Microbiological Reviews·R B Wickner
May 1, 1995·Molecular and Cellular Biology·Y Ohtake, R B Wickner
Mar 27, 2012·American Journal of Human Genetics·Alexandre FabreCatherine Badens

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.