Superoxide and endothelium-dependent constriction to flow in porcine small pulmonary arteries

British Journal of Pharmacology
Q LiuN A Flavahan

Abstract

1. The aim of this study was to determine the response of porcine small pulmonary arteries to intralumenal flow and to identify the cellular mechanisms and potential mediators involved in the response. 2. Porcine small pulmonary arteries were isolated from a branch of the main intrapulmonary artery of the lower lung lobe and studied in a perfusion myograph system that allowed independent control of transmural pressure and intralumenal flow. At a transmural pressure of 20 mmHg, the baseline internal diameter (BID) of the arteries was 251.2+/-16.1 microm (n=16). 3. Under quiescent conditions or during constriction with U46619 to approximately 60% of BID, intralumenal flow caused reversible constriction in arteries with endothelium (in the presence of U46619, flow decreased diameter from 60.0+/-2.5% to 49.5+/-3.0% BID at 10 microl min(-1), n=16, P<0.05) but no change in diameter of arteries without endothelium. 4. In the presence of superoxide dismutase (SOD, 150 u ml(-1)), the response to flow was converted from constriction to vasodilatation (in presence of U46619 and SOD, flow increased diameter from 54.2+/-3.4% to 76.7+/-4.5% BID at 10 microl min(-1), n=10, P<0.05). Inhibition of NO synthase with L-NAME (3 x 10(-5) M) abolishe...Continue Reading

References

Oct 23, 1992·Science·R C ZiegelsteinM C Capogrossi
May 1, 1990·Circulation Research·J L Garcia-Roldan, J A Bevan
Jul 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·N Resnick, M A Gimbrone
Jul 1, 1995·Physiological Reviews·P F Davies
Jan 1, 1993·Journal of Cardiovascular Pharmacology·R M NeremR W Alexander
Jun 15, 1994·Biochemical and Biophysical Research Communications·Q LanP F Davies
Mar 1, 1994·The American Journal of Physiology·M J Kuchan, J A Frangos
Jan 1, 1993·Annals of Biomedical Engineering·B R AlevriadouW P Schilling
Jan 1, 1996·Free Radical Biology & Medicine·Z S Katusic
Feb 1, 1997·British Journal of Pharmacology·Q Liu, N A Flavahan

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Citations

Feb 9, 2000·Pharmacology & Therapeutics·A T Demiryürek, R M Wadsworth
Dec 25, 2002·Trends in Pharmacological Sciences·Rudi Busse, Ingrid Fleming
Feb 4, 2009·Antioxidants & Redox Signaling·Hanke L MatlungEd VanBavel
Feb 19, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ok-Nam BaeJin-Ho Chung
Sep 23, 2008·Free Radical Biology & Medicine·Ghazaleh Esmaeil PourmahramJeremy P T Ward
Jul 1, 2008·Pharmacology & Therapeutics·Daphne MerkusDirk J Duncker
Apr 28, 2012·British Journal of Pharmacology·John Craig, William Martin
Feb 5, 2002·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Tsai-Fwu ChouChau-Fong Chen
Mar 17, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·John Q Liu, Rodney J Folz
Dec 22, 1999·The American Journal of Physiology·J A Madden, N J Christman
Jul 5, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Eva Nozik-GrayckClaude A Piantadosi
May 18, 1999·The American Journal of Physiology·Q Liu, J T Sylvester

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