Superoxide and its metabolism during germination and axis growth of Vigna radiata (L.) Wilczek seeds

Plant Signaling & Behavior
Khangembam Lenin SinghRup Kumar Kar

Abstract

Involvement of reactive oxygen species in regulation of plant growth and development is recently being demonstrated with various results depending on the experimental system and plant species. Role of superoxide and its metabolism in germination and axis growth was investigated in case of Vigna radiata seeds, a non-endospermous leguminous species having epigeal germination, by studying the effect of different reactive oxygen species (ROS) inhibitors, distribution of O2(•)- and H2O2 and ROS enzyme profile in axes. Germination percentage and axis growth were determined under treatment with ROS inhibitors and scavengers. Localization of O2(•)- and H2O2 was done using nitroblue tetrazolium (NBT) and 3,3',5,5'-tetramethyl benzidine dihydrochloride hydrate (TMB), respectively. Apoplastic level of O2(•)- was monitored by spectrophotometric analysis of bathing medium of axes. Profiles of NADPH oxidase and superoxide dismutase (SOD) were studied by in-gel assay. Germination was retarded by treatments affecting ROS level except H2O2 scavengers, while axis growth was retarded by all. Superoxide synthesis inhibitor and scavenger prevented H2O2 accumulation in axes in later phase as revealed from TMB staining. Activity of Cu/Zn SOD1 was ini...Continue Reading

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Citations

Oct 28, 2019·The Biochemical Journal·Christophe Bailly
Dec 6, 2020·International Journal of Molecular Sciences·Natalia WojciechowskaEwa M Kalemba
Mar 3, 2019·Plant Science : an International Journal of Experimental Plant Biology·Linda De BontFrançois Bouteau

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