Abstract
Reactive oxygen species play an important role in promoting inflammation. Blood monocytes have been described to release higher amounts of superoxide anion in uncontrolled and untreated asthmatics. Corticosteroids are widely used in asthma, but little is known about their molecular mechanism of action. The aim of our study was to analyse the ex vivo effect of corticosteroid treatments on superoxide anion release by blood monocytes. Superoxide anion release was measured by a spectrophotometric method based on the superoxide dismutase (SOD) -inhibitable reduction of ferricytochrome C by blood monocytes from untreated patients and asthmatics treated with i.v., inhaled and oral corticosteroids. Monocytes from uncontrolled and untreated asthmatics, released high amounts of superoxide anions. After short-term treatment with i.v., corticosteroids, this release was found to decrease significantly (1.410 versus 0.340 nM, p<0.05). Cells from asthmatics who had undergone long-term treatment with inhaled or oral steroids presented low amounts of superoxide anion production, with a significant difference as compared to untreated asthmatics (0.375 nM p<0.01 and 0.620 nM p<0.02 respectively). In general, patients with controlled asthma (treat...Continue Reading
Citations
Dec 31, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Ning LiAndre E Nel
Feb 21, 2006·Antioxidants & Redox Signaling·Ning Li, Andre E Nel
Dec 27, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Marta PerezRobin H Steinhorn
Jun 5, 2002·American Journal of Respiratory and Critical Care Medicine·Caroline BonnansPascal Chanez
May 11, 2005·American Journal of Respiratory and Critical Care Medicine·Suzy A A ComhairSerpil C Erzurum
Jan 18, 2014·Journal of Medicinal Food·Maria T MonforteEnza M Galati
Oct 31, 2015·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Amel Ben AnesKarim Chahed
Jan 19, 2011·Allergologia et immunopathologia·Ece OnurHasan Yüksel
Dec 6, 2006·Steroids·A M SadowskaW A De Backer
May 26, 2004·Immunology Letters·Janusz BednarekJerzy Sowiński
Jul 1, 2006·Environmental Toxicology and Pharmacology·Süleyman KaleliFatih Gültekin
Aug 17, 2014·Toxicology Letters·Ruchi RoyPremendra D Dwivedi
Aug 28, 1999·Respiratory Medicine·P Tarodo de la FuenteP Chanez
Aug 11, 2011·International Archives of Allergy and Immunology·Li-Ling YangTsu-Nai Wang
Feb 22, 2003·Pediatric Research·Bettina C SchockMadeleine Ennis
Jul 11, 2006·Retina·José Maria Ruiz-MorenoMarco A Zarbin
Feb 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael J WhitekusAndre E Nel
Sep 16, 2010·Molecular and Cellular Biochemistry·Krishan Lal KhandujaDegambar Behera
Jun 9, 2006·European Journal of Ophthalmology·J M Ruiz-MorenoS Barile
Oct 1, 2011·The World Allergy Organization Journal·Umit M SahinerOmer Kalayci
May 10, 2001·The Journal of Allergy and Clinical Immunology·I VachierP Chanez