Superoxide Anion Production by the Spermatozoa of Men with Varicocele: Relationship with Varicocele Grade and Semen Parameters

The World Journal of Men's Health
Ilter AlkanMehmet Murad Başar

Abstract

To investigate the pathophysiological role of superoxide anion and total reactive oxygen species (ROS) production by the spermatozoa of men with varicocele and its relationship with varicocele grade and semen parameters. This prospective study included 34 men with grade II-III varicocele, regardless of their fertility status. The control group consisted of 13 healthy men. Semen characteristics were examined according to the 2010 World Health Organization criteria. The swim-up method was used for sperm preparation. Total ROS and superoxide anion production was assayed by luminol- and lucigenin-dependent chemiluminescence (CL), respectively. The men with varicocele had significantly higher total ROS and superoxide anion levels than the healthy control subjects (2.9±0.4 relative light unit (RLU) vs. 2.4±0.1 RLU, p=0.001 for luminol-dependent CL and 2.8±0.4 RLU vs. 2.3±0.2 RLU, p=0.002 for lucigenin-dependent CL). Cases of grade III varicocele had significantly higher superoxide anion and total ROS levels than grade II cases and control subjects (p<0.001). Superoxide anion and total ROS levels were negatively correlated with all semen parameters. The superoxide anion levels produced by spermatozoa were significantly higher in varic...Continue Reading

References

Jul 1, 1989·Biology of Reproduction·R J AitkenS Fishel
Oct 1, 1994·Fertility and Sterility·F MazzilliF Dondero
Feb 1, 1993·Free Radical Biology & Medicine·E de Lamirande, C Gagnon
Jan 1, 1997·Reviews of Reproduction·E de LamirandeC Gagnon
Jun 13, 1998·The Journal of Biological Chemistry·J DuranteauP T Schumacker
Sep 15, 2001·Human Reproduction Update·C K NaughtonA Agarwal
Jul 29, 2004·DNA Repair·Ari Barzilai, Ken-Ichi Yamamoto
Feb 26, 2008·BJU International·Yuichi SakamotoMasato Fujisawa
Jan 27, 2009·Urology·Ashok AgarwalEdmund Sabanegh
Nov 1, 2011·Fertility and Sterility·Armand Zini, Gert Dohle
Oct 7, 2015·Cell Metabolism·M Carmen Fernández-AgüeraJosé López-Barneo
Jun 9, 2017·Redox Biology·Kimberly A SmithPaul T Schumacker

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Citations

Apr 10, 2020·Andrologia·Saradha BaskaranRalf Henkel
Aug 31, 2020·International Urology and Nephrology·Meysam SalehzadehSeyed Morteza Bagheri
Jul 3, 2021·International Journal of Environmental Research and Public Health·Kamil GillMalgorzata Piasecka

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