PMID: 40548Jul 15, 1979

Superoxide dismutase, catalase and scavengers of hydroxyl radical protect against the toxic action of alloxan on pancreatic islet cells in vitro

The Biochemical Journal
K GrankvistI B Täljedal

Abstract

Experiments with isolated pancreatic islets or dispersed islet cells from non-inbred ob/ob mice were performed to test the hypothesis that free radicals, notably OH., mediate the diabetogenic toxicity of alloxan. Accumulation of 86Rb+ by whole islets and exclusion of Trypan Blue by dispersed cells were used as previously validated criteria of islet-cell viability. Alloxan alone drastically inhibited the Rb+ accumulation and significantly decreased the frequency of cells excluding Trypan Blue. Enzymic scavengers of O2.- and H2O2 or non-enzymic scavengers of OH. or singlet oxygen were added to the incubation medium and tested for their ability to protect against these effects of alloxan. Superoxide dismutase, catalase, dimethyl sulphoxide, benzoate, and mannitol counteracted the effects of alloxan in both cytotoxicity assays. Significant protection of the Rb+-accumulating capacity was also afforded by butanol, caffeine, theophylline, NADH, NADPH and, to a small extent, NAD+. Urea has a poor affinity for OH. and did not protect against alloxan. No effect was obtained with the singlet-oxygen scavenger, histidine. Except for the protection by NADH and NADPH, which may be due to a direct reaction with alloxan in the medium, the resul...Continue Reading

Citations

Jan 1, 1991·Free Radical Research Communications·T IshiiR Kido
Jan 1, 1984·Critical Reviews in Toxicology·J DiGuiseppi, I Fridovich
Jan 1, 1983·Drug Metabolism Reviews·K E Mc LaneK Ramakrishnan
Dec 18, 2003·Annals of the New York Academy of Sciences·Kathryn HaskinsJennifer Kench
Dec 1, 1989·Toxicology and Applied Pharmacology·D G Scarpelli
Dec 1, 1984·Experimental and Molecular Pathology·R K CrouchJ A Simson
Jan 1, 1989·Fundamental & Clinical Pharmacology·D Bentue-FerrerJ Van den Driessche
Jan 1, 1985·Acta Paediatrica Scandinavica. Supplement·S Sandler, N Welsh
Nov 22, 2005·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M M Sailaja Devi, Undurti N Das
Dec 1, 1992·Biochemical Pharmacology·J M Edwardson, P M Dean
Jan 1, 1986·American Journal of Surgery·K S GuiceJ C Thompson
Aug 26, 2003·Redox Report : Communications in Free Radical Research·Mohammad IqbalShigeru Okada
Feb 19, 2008·Acta Biologica Hungarica·Albena AlexandrovaMargarita Kirkova
Nov 15, 2011·Molecular and Cellular Endocrinology·Ahmet KorkmazRussel J Reiter
Dec 29, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Sadiq S MoreeJ Rajesha
Nov 30, 2010·Diabetes Research and Clinical Practice·R G Ahmed
Nov 16, 2004·The Journal of Nutritional Biochemistry·P VenkataramanJ Arunakaran
Nov 1, 1991·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H ZhangU T Brunk
Dec 22, 2014·Archives of Biochemistry and Biophysics·Akira UrunoMasayuki Yamamoto
Nov 12, 1981·Nature·K GrankvistI B Täljedal
Jan 18, 2006·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Y Suresh, Undurti N Das
Jul 29, 1983·Biochemical and Biophysical Research Communications·I Domke, W Weis
Aug 1, 1983·Biological Trace Element Research·J R SorensonA Pezeshk
Aug 21, 2001·Biological & Pharmaceutical Bulletin·K SakuraiY Fujimoto
Sep 9, 2008·Antioxidants & Redox Signaling·John J MieyalMelissa D Shelton
Dec 1, 1988·Molecular and Cellular Biochemistry·D V GodinA D Goumeniouk
Dec 1, 1984·Diabetologia·G L WilsonB T Mossman
Dec 1, 1989·Die Naturwissenschaften·M NukatsukaJ Kawada
Jun 15, 1992·FEBS Letters·S KojimaK Kubota
Jan 1, 1984·Virchows Archiv. A, Pathological Anatomy and Histopathology·R NorlundI B Täljedal
Oct 12, 1981·FEBS Letters·F Malaisse-LagaeW J Malaisse
Jul 1, 1985·Medicinal Research Reviews·I A ClarkN H Hunt
Sep 1, 1979·FEBS Letters·K GrankvistI B Täljedal
Dec 1, 1985·Environmental Health Perspectives·G RotilioM R Ciriolo
Jul 1, 1992·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·H A LehrK Messmer
Oct 1, 1990·Acta diabetologica latina·N SekarS Govindasamy
Jan 1, 1987·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·P Flament, C Remacle
Jun 1, 1986·Journal of Neurocytology·L G ThaeteS S Spicer
Sep 27, 2008·Pakistan Journal of Biological Sciences : PJBS·S R Fatemi TabatabaeiL Rahimi
Nov 1, 1988·Pharmacology & Toxicology·D L Eizirik, S Sandler
Apr 1, 1992·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H ZhangU T Brunk
Jan 1, 1986·Virchows Archiv. A, Pathological Anatomy and Histopathology·L Jansson, S Sandler
Jul 1, 1981·Diabetologia·I B Täljedal
Dec 31, 2011·Applied Biochemistry and Biotechnology·Verônica B BritoJoão Batista T Rocha
Oct 29, 2005·Endocrinology·Hongjun WangFritz H Bach
Feb 1, 1992·Acta Pathologica Japonica·R SatoI Nakayama
Nov 10, 2018·Scientific Reports·Kuan-Ting LeeShih-Yuan Lu

Related Concepts

Alloxan
Catalase T
Free Radicals
Islets of Langerhans
NADH
NADP
Oxidation-Reduction
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