PMID: 9529045Apr 7, 1998Paper

Superoxide dismutase-dependent, catalase-sensitive peroxides in human endothelial cells infected by Rickettsia rickettsii

Infection and Immunity
J E HongD J Silverman

Abstract

The generation and intracellular accumulation of reactive oxygen species have been shown to be associated with the infection of human umbilical vein endothelial cells (HUVEC) by Rickettsia rickettsii. In response to the oxidant superoxide, the activity of the enzyme superoxide dismutase (SOD) increases following infection by this obligate intracellular bacterium. Other oxidants which are capable of oxidizing the fluorescent probe 2',7'-dichlorofluorescin (DCFH) also accumulate intracellularly within infected cells. In the study reported here, we show that (i) an inhibitor of SOD, diethyldithiocarbamic acid, reduces the observed rise in SOD activity in infected cells by 40 to 60% and at the same time reduces the degree of intracellular oxidation of DCFH; (ii) catalase-sensitive peroxides can be detected in supernatants of R. rickettsii-infected cells shortly after rickettsial exposure; and (iii) fluorescence-activated cell sorter analysis demonstrates significant intracellular oxidant activity in infected cells within 5 h after exposure to R. rickettsii. The results of these experiments indicate that hydrogen peroxide is a major oxidant associated with infection of HUVEC by R. rickettsii and that intracellular oxidant activity s...Continue Reading

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Citations

Jun 22, 2010·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Hana BártíkováBarbora Szotáková
Mar 5, 2014·Transboundary and Emerging Diseases·M P La MannaG Sireci
Oct 14, 2016·American Journal of Physiology. Cell Physiology·Geoffrey E Ciarlone, Jay B Dean
Jul 16, 2003·Annals of the New York Academy of Sciences·David H WalkerJuan P Olano
Mar 26, 2009·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·L ZastrowL Ferrero
Mar 27, 2007·Revista Do Instituto De Medicina Tropical De São Paulo·Vanuza Cristina SáAnamélia Lorenzetti Bocca
Feb 17, 2006·Annals of the New York Academy of Sciences·Juan P Olano
Jul 13, 2017·International Journal of Molecular Sciences·Abha SahniSanjeev K Sahni
Nov 30, 2000·Microbial Pathogenesis·E ValenteM C Plotkowski

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