Superoxide dismutase reduces the inflammatory response to Aspergillus and Alternaria in human sinonasal epithelial cells derived from patients with chronic rhinosinusitis

American Journal of Rhinology & Allergy
Lauren A LawrenceRodney J Schlosser

Abstract

Aspergillus fumigatus and Alternaria alternata are ubiquitous environmental fungal allergens that can exacerbate airway inflammation and contribute to the disease process in patients with chronic rhinosinusitis (CRS). These antigens have been shown to induce human sinonasal epithelial cells (HSNECs) to promote a proinflammatory response, but what is unclear is a means by which to reduce these effects. Inhaled pathogens can induce HSNECs to produce reactive oxygen species (ROS) that trigger cytokine production. This study aimed to determine whether the free radical scavenger superoxide dismutase (SOD) could reduce HSNEC-derived inflammation, as measured by interleukin (IL)-6 and IL-8 production, in response to Aspergillus or Alternaria exposure. Sinus tissue explants were collected at the time of surgery from control patients (n = 7) and patients with CRS with nasal polyps (CRSwNP) (n = 9). HSNECs were cultured from the explants and treated with Aspergillus, Alternaria, and SOD for 24 hours. Cell supernatants and lysates were collected, and IL-6 and IL-8 concentrations were measured using enzyme-linked immunosorbent assay. In control and CRSwNP HSNECs, Aspergillus and Alternaria both increased cytokine production (p < 0.05), as ...Continue Reading

References

Sep 1, 1978·Clinical Allergy·J Mullins, A Seaton
Sep 1, 1975·The Journal of Allergy and Clinical Immunology·W R Solomon
Jul 1, 1994·Free Radical Biology & Medicine·C FeldmanR Wilson
Oct 6, 1997·The European Respiratory Journal·L D MartinK B Adler
Apr 9, 1998·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·A H Assa'adM B Lierl
Mar 9, 1999·The Journal of Allergy and Clinical Immunology·C RhyooD Proud
Sep 17, 1999·Mayo Clinic Proceedings·J U PonikauG D Roberts
May 5, 2001·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·H DoğruK Uygur
Jan 10, 2002·Pulmonary Pharmacology & Therapeutics·P A Henricks, F P Nijkamp
Jul 20, 2002·Free Radical Biology & Medicine·Ling-Yi Chang, James D Crapo
Feb 5, 2003·The Laryngoscope·Hannes BraunHeinz Stammberger
Feb 13, 2003·Current Opinion in Allergy and Clinical Immunology·Ruby Pawankar
Jul 6, 2004·The Laryngoscope·Muharrem DagliHakan Korkmaz
Dec 4, 2004·The Journal of Allergy and Clinical Immunology·Seung-Heon ShinHirohito Kita
Oct 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yoshinari InoueHirohito Kita
Feb 7, 2006·Pharmacology & Therapeutics·Paul Kirkham, Irfan Rahman
Jan 10, 2009·Advanced Drug Delivery Reviews·Samuel K LaiJustin Hanes
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takao KobayashiHirohito Kita
Feb 18, 2010·The Journal of Allergy and Clinical Immunology·Anju T PetersRobert P Schleimer
Apr 7, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·David W DenningDavid S Perlin
Jan 31, 2012·International Forum of Allergy & Rhinology·Jennifer K MulliganRodney J Schlosser
Nov 22, 2012·American Journal of Rhinology & Allergy·Zachary M Soler, Rodney J Schlosser
May 22, 2013·Mediators of Inflammation·I-Ta Lee, Chuen-Mao Yang
Apr 5, 2014·The Journal of Allergy and Clinical Immunology·Jennifer K MulliganRodney J Schlosser
Apr 11, 2014·American Journal of Rhinology & Allergy·Ozgul TopalSelim S Erbek

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Citations

Feb 3, 2016·Biochemistry·Cigall KadochHeike Keilhack
Jun 3, 2017·International Forum of Allergy & Rhinology·Jennifer K MulliganRodney J Schlosser
May 19, 2017·The Biochemical Journal·Yong-Sung ParkCheol-Won Yun
Jun 17, 2018·Mucosal Immunology·Jennifer K MulliganCarl Atkinson

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Methods Mentioned

BETA
Assay
enzyme-linked immunosorbent assay
Protein Assay

Software Mentioned

GraphPad
GraphPad Prism

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