Superoxide-hydrogen peroxide genetic imbalance modulates differentially the oxidative metabolism on human peripheral blood mononuclear cells exposed to seleno-L-methionine

Chemico-biological Interactions
Karen Lilian SchottIvana Beatrice Mânica da Cruz

Abstract

Superoxide-hydrogen peroxide (S-HP) imbalance genetically caused by a gene polymorphism in the human manganese superoxide dismutase enzyme (Val16Ala-MnSOD) is associated with several diseases. Into mitochondria, MnSOD catalyses superoxide radical producing HP and oxygen. Ala-MnSOD genotype presents a high MnSOD efficiency and generates the highest HP concentrations that has been associated with the risk of several cancer types. Cellular selenoenzymes glutathione peroxidase and thioredoxin reductase (TrxR) and catalase (CAT) are essential to HP removal produced in excess in cells. Since, synthesis and activities of selenoenzymes are selenium dependent, we hypothesized that AA-MnSOD cells could have an improvement on antioxidant status undergoing Seleno-L-methionine (SeMet) treatment. This study performed an in vitro protocol to evaluate the response of peripheral blood mononuclear cells (PBMC) carriers of different Val16Ala-MnSOD genotypes exposed to SeMet. SeMet effects on cell viability, apoptosis induction and modulation of oxidative variables were determined using spectrophotometric, flow cytometry, fluorimetric and immunoassays. Gene modulation of antioxidant enzymes was also performed by qRT-PCR. From an initial protocol u...Continue Reading

References

Jun 14, 2008·The British Journal of Nutrition·Raxit J JariwallaDaniel Nakamura
May 26, 2009·Biochimica Et Biophysica Acta·Zoia R Stoytcheva, Marla J Berry
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Citations

Dec 19, 2017·BioMed Research International·Fernanda BarbisanIvana Beatrice Mânica da Cruz
Jul 1, 2020·Journal of Medicinal Food·Greice Franciele Feyh Dos Santos MontagnerIvana Beatrice Mânica da Cruz
Nov 16, 2020·Chemosphere·Ivo Emilio da Cruz JungIvana Beatrice Mânica da Cruz
May 31, 2021·Neurochemistry International·Charles Elias AssmannIvana Beatrice Mânica da Cruz
Sep 24, 2018·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Karen Lilian SchottIvana Beatrice Mânica da Cruz

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