PMID: 15383592Sep 24, 2004Paper

Superoxide production at phagosomal cup/phagosome through beta I protein kinase C during Fc gamma R-mediated phagocytosis in microglia

The Journal of Immunology : Official Journal of the American Association of Immunologists
Takehiko UeyamaN Saito

Abstract

Protein kinase C (PKC) plays a prominent role in immune signaling. To elucidate the signal transduction in a respiratory burst and isoform-specific function of PKC during FcgammaR-mediated phagocytosis, we used live, digital fluorescence imaging of mouse microglial cells expressing GFP-tagged molecules. betaI PKC, epsilonPKC, and diacylglycerol kinase (DGK) beta dynamically and transiently accumulated around IgG-opsonized beads (BIgG). Moreover, the accumulation of p47(phox), an essential cytosolic component of NADPH oxidase and a substrate for betaI PKC, at the phagosomal cup/phagosome was apparent during BIgG ingestion. Superoxide (O(2)(-)) production was profoundly inhibited by Gö6976, a cPKC inhibitor, and dramatically increased by the DGK inhibitor, R59949. Ultrastructural analysis revealed that BIgG induced O(2)(-) production at the phagosome but not at the intracellular granules. We conclude that activation/accumulation of betaI PKC is involved in O(2)(-) production, and that O(2)(-) production is primarily initiated at the phagosomal cup/phagosome. This study also suggests that DGKbeta plays a prominent role in regulation of O(2)(-) production during FcgammaR-mediated phagocytosis.

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Citations

Oct 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tracy CrottyMatthew K Topham
May 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Deepak Yadav, Nora Sarvetnick
Aug 6, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takehiko UeyamaNaoaki Saito
Apr 14, 2010·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Reiko MinakamiHideki Sumimoto
Oct 22, 2009·Neuromolecular Medicine·Eitan OkunThiruma V Arumugam
Nov 28, 2013·Scientifica·Carlos Barcia
Dec 17, 2014·Acta Neuropathologica·Valérie JolivelMirko H H Schmidt
Apr 9, 2016·Cellular Logistics·Shuwei XieSteve Caplan
Mar 11, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Luke M HealyJack P Antel
Jun 9, 2016·Chembiochem : a European Journal of Chemical Biology·Eric Lindberg, Nicolas Winssinger
Sep 20, 2020·International Journal of Molecular Sciences·Fumio SakaneChiaki Murakami
Jun 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takehiko UeyamaNaoaki Saito
Oct 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Virginia L Vega, Antonio De Maio
May 15, 2012·Neuroreport·Bart R TambuyzerChris Van Ginneken
May 15, 2009·Antioxidants & Redox Signaling·Thomas L LetoTakehiko Ueyama
Aug 7, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takehiko UeyamaNaoaki Saito
Nov 24, 2006·Molecular Biology of the Cell·Takehiko UeyamaNaoaki Saito
May 15, 2012·Cellular and Molecular Life Sciences : CMLS·Michael J Surace, Michelle L Block
Feb 23, 2021·Frontiers in Cell and Developmental Biology·Fernando Montaño-RendónGlenn F W Walpole
Apr 13, 2021·Frontiers in Cellular Neuroscience·Thomas Ernest James Phillips, Emily Maguire
May 22, 2007·Biochimica Et Biophysica Acta·Fumio SakaneHideo Kanoh
Mar 6, 2009·Immunobiology·Ann M Kerrigan, Gordon D Brown

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