Superoxide reductase from the syphilis spirochete Treponema pallidum: crystallization and structure determination using soft X-rays

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Teresa Santos-SilvaMaria João Romão

Abstract

Superoxide reductase is a 14 kDa metalloprotein containing a catalytic non-haem iron centre [Fe(His)4Cys]. It is involved in defence mechanisms against oxygen toxicity, scavenging superoxide radicals from the cell. The oxidized form of Treponema pallidum superoxide reductase was crystallized in the presence of polyethylene glycol and magnesium chloride. Two crystal forms were obtained depending on the oxidizing agents used after purification: crystals grown in the presence of K3Fe(CN)6 belonged to space group P2(1) (unit-cell parameters a = 60.3, b = 59.9, c = 64.8 A, beta = 106.9 degrees) and diffracted beyond 1.60 A resolution, while crystals grown in the presence of Na2IrCl6 belonged to space group C2 (a = 119.4, b = 60.1, c = 65.6 A, beta = 104.9 degrees) and diffracted beyond 1.55 A. A highly redundant X-ray diffraction data set from the C2 crystal form collected on a copper rotating-anode generator (lambda = 1.542 A) clearly defined the positions of the four Fe atoms present in the asymmetric unit by SAD methods. A MAD experiment at the iron absorption edge confirmed the positions of the previously determined iron sites and provided better phases for model building and refinement. Molecular replacement using the P2(1) dat...Continue Reading

References

Feb 1, 1992·Acta Crystallographica. Section B, Structural Science·Z DauterK S Wilson
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Jan 1, 1995·Annual Review of Biochemistry·I Fridovich
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
May 20, 1999·Nature Structural Biology·A PerrakisV S Lamzin
May 26, 1999·Journal of Molecular Biology·Z DauterG M Sheldrick
Sep 22, 2001·Acta Crystallographica. Section D, Biological Crystallography·R J Read
Mar 3, 2004·Acta Crystallographica. Section D, Biological Crystallography·Laurent C StoroniRandy J Read

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Citations

May 18, 2011·BMC Microbiology·Céline Lucchetti-MiganehFrédérique Barloy-Hubler
Jun 23, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Teresa Santos-SilvaMaria João Romão

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