Superparamagnetic iron oxide nanoparticles labeling of bone marrow stromal (mesenchymal) cells does not affect their "stemness".

PloS One
Arun BalakumaranPamela Gehron Robey

Abstract

Superparamagnetic iron oxide nanoparticles (SPION) are increasingly used to label human bone marrow stromal cells (BMSCs, also called "mesenchymal stem cells") to monitor their fate by in vivo MRI, and by histology after Prussian blue (PB) staining. SPION-labeling appears to be safe as assessed by in vitro differentiation of BMSCs, however, we chose to resolve the question of the effect of labeling on maintaining the "stemness" of cells within the BMSC population in vivo. Assays performed include colony forming efficiency, CD146 expression, gene expression profiling, and the "gold standard" of evaluating bone and myelosupportive stroma formation in vivo in immuncompromised recipients. SPION-labeling did not alter these assays. Comparable abundant bone with adjoining host hematopoietic cells were seen in cohorts of mice that were implanted with SPION-labeled or unlabeled BMSCs. PB+ adipocytes were noted, demonstrating their donor origin, as well as PB+ pericytes, indicative of self-renewal of the stem cell in the BMSC population. This study confirms that SPION labeling does not alter the differentiation potential of the subset of stem cells within BMSCs.

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Citations

Oct 24, 2013·Stem Cells and Development·Saejeong KimJoseph A Frank
Mar 22, 2014·Chemical Society Reviews·Yi ZhangBing Yan
Jul 19, 2014·Stem Cells and Development·Nikolaos PapadimitriouHelena Barreto Henriksson
Apr 24, 2012·Stem Cell Research & Therapy·Ian WimpennyAlicia J El Haj
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Oct 23, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yingxun Liu, Jinke Wang
Oct 11, 2013·Nanoscale·Roxanne HachaniNguyen Thi Kim Thanh
Aug 30, 2014·International Journal of Environmental Research and Public Health·Caterina FedeStefano Cagnin
Feb 24, 2015·Scandinavian Cardiovascular Journal : SCJ·Kirsi AlestaloPetri Lehenkari
Aug 4, 2015·Contrast Media & Molecular Imaging·Saejeong J KimJoseph A Frank
Jan 21, 2014·Academic Radiology·Sheng-Min LanRuey-Mo Lin
Dec 12, 2012·Nanomedicine : Nanotechnology, Biology, and Medicine·Dickson K KiruiCarl A Batt
Mar 28, 2016·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Olli-Pekka LappalainenGeorge K B Sándor
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Dec 23, 2015·Annals of Biomedical Engineering·Daniela Y SantiestebanStanislav Emelianov

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Methods Mentioned

BETA
imaging techniques
flow cytometry
electron microscopy
biopsies
light microscopy
transfection
dissection
FACS

Software Mentioned

MicroSuite⢠Biological Suite
Axiovision
CellQuest
Cluster
Treeview
BRBArrayTools

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