Superresolution 4π Raman microscopy

Optics Letters
Alejandro Diaz TormoNicolas Le Thomas

Abstract

The advent of 4π microscopy broke the conventional optical resolution limit in the axial direction of the microscope. In combination with fluorescence microscopy, it broadened the knowledge of cell biology at the expense of perturbing the samples with extrinsic fluorescent labels. In contrast, Raman microscopy acquires the molecular fingerprint of the sample without the need of extrinsic labels, and therefore improving its resolution can make an even greater impact. Here, we take advantage of the improved axial resolution of a 4π configuration to form a 4π Raman microscope. With this microscope, we independently and simultaneously analyzed different nanolayers in a multilayer stack. We identified their chemical composition and retrieved their relative subwavelength optical separation with a precision of 6 nm.

References

Mar 5, 2004·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Jörg SchillingErich Sackmann
May 26, 2007·Science·Stefan W Hell
Apr 8, 2010·Optics Express·Seok-Beom Cho, Tae-Gon Noh
Nov 28, 2014·Analytical and Bioanalytical Chemistry·Christoph Krafft, Jürgen Popp
Sep 30, 2015·Nature Communications·C NeumannC Stampfer
Nov 12, 2015·Applied Spectroscopy·Lucas LangelüddeckeVolker Deckert
Dec 3, 2015·Nature Communications·Kozue WatanabeKatsumasa Fujita

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Citations

Jun 30, 2019·Analytical Chemistry·Christian T GraefeRenee R Frontiera

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