Superresolution imaging of viral protein trafficking

Medical Microbiology and Immunology
Anamaris M Colberg-PoleyJyoti K Jaiswal

Abstract

The endoplasmic reticulum (ER) membrane is closely apposed to the outer mitochondrial membrane (OMM), which facilitates communication between these organelles. These contacts, known as mitochondria-associated membranes (MAM), facilitate calcium signaling, lipid transfer, as well as antiviral and stress responses. How cellular proteins traffic to the MAM, are distributed therein, and interact with ER and mitochondrial proteins are subject of great interest. The human cytomegalovirus UL37 exon 1 protein or viral mitochondria-localized inhibitor of apoptosis (vMIA) is crucial for viral growth. Upon synthesis at the ER, vMIA traffics to the MAM and OMM, where it reprograms the organization and function of these compartments. vMIA significantly changes the abundance of cellular proteins at the MAM and OMM, including proteins that regulate calcium homeostasis and cell death. Through the use of superresolution imaging, we have shown that vMIA is distributed at the OMM in nanometer scale clusters. This is similar to the clusters reported for the mitochondrial calcium channel, VDAC, as well as electron transport chain, translocase of the OMM complex, and mitochondrial inner membrane organizing system components. Thus, aside from address...Continue Reading

References

Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·V S GoldmacherT Chittenden
Aug 12, 2000·The Journal of Biological Chemistry·S J Stone, J E Vance
Oct 19, 2000·The Journal of Cell Biology·S KanajiK Mihara
Apr 17, 2002·Physical Review Letters·Marcus Dyba, Stefan W Hell
Feb 11, 2004·The Journal of General Virology·Manohara S Mavinakere, Anamaris M Colberg-Poley
Aug 10, 2006·Nature Methods·Michael J RustXiaowei Zhuang
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Sep 20, 2006·The Journal of Cell Biology·György CsordásGyörgy Hajnóczky
Sep 20, 2006·The Journal of Cell Biology·Delphine PoncetGuido Kroemer
Oct 14, 2006·Science·Veit HornungGunther Hartmann
Dec 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ronit Sharon-FrilingThomas Shenk
Dec 19, 2006·PLoS Pathogens·Joshua MungerJoshua D Rabinowitz
Dec 21, 2006·The Journal of Cell Biology·György SzabadkaiRosario Rizzuto
Apr 18, 2008·Journal of Virology·Kristi L Norris, Richard J Youle
Aug 7, 2008·Journal of Bioenergetics and Biomembranes·John G Pastorino, Jan B Hoek
Sep 17, 2008·Nature Methods·Jonas FöllingStefan W Hell
Dec 5, 2008·Nature·Olga Martins de Brito, Luca Scorrano
Jan 16, 2009·Trends in Cell Biology·Teruo HayashiTsung-Ping Su
Nov 27, 2009·Journal of Virology·Jeremy W ChambersJames C Alwine
Dec 19, 2009·EMBO Reports·Céline CastanierDamien Arnoult
Jul 6, 2010·Biochimica Et Biophysica Acta·Jovana Dukanovic, Doron Rapaport

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.