Superresolution microscopy with transient binding

Current Opinion in Biotechnology
Julia MollePhilip Tinnefeld

Abstract

For single-molecule localization based superresolution, the concentration of fluorescent labels has to be thinned out. This is commonly achieved by photophysically or photochemically deactivating subsets of molecules. Alternatively, apparent switching of molecules can be achieved by transient binding of fluorescent labels. Here, a diffusing dye yields bright fluorescent spots when binding to the structure of interest. As the binding interaction is weak, the labeling is reversible and the dye ligand construct diffuses back into solution. This approach of achieving superresolution by transient binding (STB) is reviewed in this manuscript. Different realizations of STB are discussed and compared to other localization-based superresolution modalities. We propose the development of labeling strategies that will make STB a highly versatile tool for superresolution microscopy at highest resolution.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E WulfT Wieland
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L S BarakW W Webb
Aug 17, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N Panchuk-VoloshinaR P Haugland
Mar 17, 2006·Nature·Paul W K Rothemund
Aug 10, 2006·Nature Methods·Michael J RustXiaowei Zhuang
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Sep 19, 2006·Biophysical Journal·Samuel T HessMichael D Mason
Dec 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Alexey Sharonov, Robin M Hochstrasser
Dec 13, 2007·Optics Letters·T A Klar, S W Hell
Jan 8, 2008·Nature Methods·Makio TokunagaKumiko Sakata-Sogawa
Jul 23, 2008·Angewandte Chemie·Mike HeilemannMarkus Sauer
Nov 13, 2008·Nature Reviews. Molecular Cell Biology·Marta Fernández-Suárez, Alice Y Ting
Oct 16, 2009·Angewandte Chemie·Christian SteinhauerPhilip Tinnefeld
Jul 16, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Jan VogelsangPhilip Tinnefeld
Aug 18, 2010·Biophysical Journal·Gregory GiannoneLaurent Cognet
Dec 31, 2010·Nature Methods·Teresa KleinMarkus Sauer
Mar 17, 2011·Journal of the American Chemical Society·Chinkuei Kuo, Robin M Hochstrasser
May 10, 2011·Nature Methods·Sara A JonesXiaowei Zhuang
Jul 2, 2011·Nature Protocols·Sebastian van de LindeMarkus Sauer
Aug 16, 2011·Nano Letters·Ingmar SchoenViola Vogel
Oct 28, 2011·Proceedings of the National Academy of Sciences of the United States of America·Matthew D LewW E Moerner
Sep 29, 2012·Nature Methods·Felipe OpazoSilvio O Rizzoli
Dec 12, 2012·Nature Methods·Jürgen J SchmiedPhilip Tinnefeld
Dec 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Brian J BeliveauChao-ting Wu
Feb 1, 2013·Nano Letters·Jürgen J SchmiedPhilip Tinnefeld
Apr 19, 2013·ACS Chemical Neuroscience·Jonas RiesLawrence Rajendran
Oct 1, 2013·ACS Applied Materials & Interfaces·Jixin ChenChristy F Landes
Mar 1, 2014·Nature Methods·Hendrik DeschoutKevin Braeckmans
Mar 8, 2014·Methods : a Companion to Methods in Enzymology·Alexander Johnson-Buck, Nils G Walter
May 17, 2014·Nature Protocols·Jürgen J SchmiedPhilip Tinnefeld
Jun 5, 2014·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Mario RaabPhilip Tinnefeld
Nov 18, 2014·Optics Express·Joran DeschampsJonas Ries
May 15, 2015·Optics Express·Susanne BeaterPhilip Tinnefeld
Sep 1, 2015·Nature Nanotechnology·Jakob Bach KnudsenKurt V Gothelf

❮ Previous
Next ❯

Citations

Aug 18, 2016·Organic & Biomolecular Chemistry·Paolo BeuzerJames J La Clair
Jun 29, 2018·Chembiochem : a European Journal of Chemical Biology·Kevin SpeharJan Bieschke
Nov 29, 2017·Beilstein Journal of Nanotechnology·Joseph R Pyle, Jixin Chen
May 13, 2019·Methods : a Companion to Methods in Enzymology·Angélique JimenezChristophe Leterrier

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.