Superwobbling facilitates translation with reduced tRNA sets

Nature Structural & Molecular Biology
Marcelo RogalskiRalph Bock

Abstract

Some bacterial and most organelle genomes do not encode the full set of 32 tRNA species required to read all codons according to Crick's wobble rules. 'Superwobble', in which a tRNA species with an unmodified U in the wobble position reads all four nucleotides in the third codon position, represents one possible mechanism for how a reduced tRNA set could still suffice. We have tested the superwobble hypothesis by producing knockout mutants for the pair of plastid glycine tRNA genes. Here we show that, whereas the tRNA gene with U in the wobble position is essential, the gene with G in this position is nonessential, demonstrating that the U-containing anticodon can indeed read all four glycine triplets. We also show that the price for superwobbling is a reduced translational efficiency, which explains why most organisms prefer pairs of isoaccepting tRNAs over the superwobbling mechanism.

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Citations

Aug 20, 2010·Plant Molecular Biology·Weimin LiRalph Bock
Mar 23, 2011·Plant Molecular Biology·Susann WickeDietmar Quandt
Jul 16, 2013·Journal of Plant Physiology·Magdalena Szechyńska-Hebda, Stanisław Karpiński
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Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andreas VerhounigRalph Bock
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May 4, 2012·Nucleic Acids Research·Sibah AlkatibRalph Bock
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