Supplemental butyrate does not enhance the absorptive or barrier functions of the isolated ovine ruminal epithelia

Journal of Animal Science
D J WilsonG B Penner

Abstract

Our objective was to determine if increasing the ruminal butyrate concentration would improve the selective permeability of ruminal epithelia. Suffolk wether lambs (n = 18) with an initial BW of 47.4 ±1.4 kg were housed in individual pens (1.5 × 1.5 m) with rubber mats on the floor. Lambs were blocked by initial BW into 6 blocks and, within block, were randomly assigned to either the control (CON) or 1 of 2 butyrate supplementation amounts (i.e., 1.25% or 2.50% butyrate as a proportion of DMI). With the exception of butyrate supplementation, all lambs were fed a common diet (90% concentrate and 10% barley silage). After a 14-d feeding period, lambs were killed, and ruminal epithelia from the ventral sac were mounted in Ussing chambers. To facilitate the Ussing chamber measurements, only 1 lamb was killed on an individual day. Thus, the starting date was staggered so that all lambs were exposed to the same experimental protocol. In Ussing chambers, epithelia were incubated using separate mucosal (pH 6.2) and serosal (pH 7.4) bathing solutions. Then 1-14C-butyrate (74 kBq/10 mL) was added to the mucosal side and was used to measure the mucosal-to-serosal flux (J(ms-butyrate)) in 2 consecutive 60-min flux periods with simultaneous...Continue Reading

References

Mar 1, 1992·Journal of Animal Science·C R KrehbielJ E Schneider
Nov 1, 1989·The American Journal of Surgical Pathology·D S Levine, R C Haggitt
Oct 1, 1987·Quarterly Journal of Experimental Physiology : an International Journal of the Physiological Society·G GaebelP Galfi
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·D GieseckeB Emmanuel
Aug 1, 1965·Journal of Dairy Science·R G Hinders, F G Owen
Jan 1, 1983·Quarterly Journal of Experimental Physiology : an International Journal of the Physiological Society·R J BarnesA Dobson
Jun 1, 1993·Zentralblatt für Veterinärmedizin. Reihe A·G GäbelH Martens
Jul 1, 1993·Journal of Dairy Science·R Britton, C Krehbiel
Feb 17, 1998·Journal of Animal Science·F N OwensD R Gill
Oct 27, 1999·Journal of Gastroenterology and Hepatology·J M MariadasonP R Gibson
Mar 10, 2000·Journal of Animal Science·J R Aschenbach, G Gäbel
Jul 18, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·J R AschenbachG Gäbel
Oct 29, 2002·Animal Health Research Reviews·G GäbelF Müller
Aug 1, 1964·The American Journal of Physiology·A F SELLERSF D MCLEOD
Nov 25, 2003·Journal of Animal Physiology and Animal Nutrition·S K UppalH Martens
Aug 21, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·C Graham, N L Simmons
Aug 26, 2004·Journal of Dairy Science·J M DeFrainD J Schingoethe
Sep 24, 2005·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Monika SchweigelHolger Martens
Dec 22, 2006·Journal of Dairy Science·G B PennerT Mutsvangwa
Mar 19, 2008·The British Journal of Nutrition·Takuya SuzukiHiroshi Hara
Mar 7, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jörg R AschenbachGotthold Gäbel
Mar 11, 2009·Archives of Animal Nutrition·Benjamin EtschmannHolger Martens
Apr 4, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lane L Clarke
Dec 22, 2009·Inflammatory Bowel Diseases·Kimberley LewisDerek M McKay

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Citations

Mar 14, 2016·Journal of Dairy Science·Michael A SteeleLe Luo Guan
Dec 30, 2016·Journal of Agricultural and Food Chemistry·Hongyu DaiXiangzhen Shen
Mar 12, 2018·Journal of Dairy Science·P GórkaP Guilloteau
Aug 1, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Brittney L SchurmannGregory B Penner
Dec 26, 2018·Journal of Dairy Science·Jörg R AschenbachGregory B Penner

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