Supplemental oxygen reverses hypoxia-induced smooth muscle cell proliferation by modulating HIF-alpha and VEGF levels in a rabbit arteriovenous fistula model

Annals of Vascular Surgery
Jing WanSteven Santilli

Abstract

Numerous mechanisms for the formation of intimal hyperplasia have been proposed but none have been proven or accepted. Our research focuses on the potential role of hypoxia-inducible factors (HIFs), vascular endothelial growth factor (VEGF), and platelet-derived growth factors as well as the extracellular signal-regulated kinase (ERK), phosphatidylinositide 3-kinase /protein Kinase B (PI3-K/AKT) pathway in hypoxia-mediated intimal hyperplasia processes. We hypothesize that HIF and VEGF will be downregulated with supplemental oxygen in our arteriovenous fistula rabbit model. Rabbits were randomized into different experimental groups with varying oxygen exposure (21% O2 or 30% O2) and receipt of surgery (surgery with fistula formation, no surgery, or sham operation with skin incision only). Plasma samples were collected at designated intervals in which cytokines and smooth muscle cell proliferation were measured. In addition, cell specimens were exposed to hyperoxic, normoxic, and hypoxic environments with cytokines measured at various time points. Placement of an arteriovenous fistula resulted in hypoxia-induced HIF stabilization with a concurrent increase in VEGF levels. There was a 4.2-fold induction in HIF-1α levels in animal...Continue Reading

References

Sep 1, 1994·The British Journal of Surgery·M G Davies, P O Hagen
Mar 3, 1999·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·S SivanesanA Bakran
May 7, 1999·Kidney International·S J SchwabD Beasley
May 19, 2000·The Journal of Surgical Research·E S LeeS M Santilli
Dec 21, 2005·Advances in Skin & Wound Care·William W LiVincent W Li
Apr 25, 2006·FEBS Letters·Himadri RoySeppo Ylä-Herttuala
Apr 25, 2006·Nature Reviews. Molecular Cell Biology·Anna-Karin OlssonLena Claesson-Welsh
Feb 14, 2008·Journal of Atherosclerosis and Thrombosis·Mayuko Osada-OkaTakashi Sato
Aug 19, 2008·Biochemistry. Biokhimii︠a︡·A F Karamysheva

❮ Previous
Next ❯

Citations

Jan 26, 2016·Kidney International·Akshaar BrahmbhattSanjay Misra
Jan 27, 2018·Cardiovascular Research·Kerry WadeySarah George
May 7, 2019·Expert Review of Cardiovascular Therapy·Mohan SatishDevendra K Agrawal
May 23, 2019·The Journal of Vascular Access·Joana Gameiro, Jose Ibeas
Nov 2, 2019·International Journal of Molecular Sciences·Nirvana SadaghianlooNathalie M Mazure
Oct 15, 2019·The Journal of Vascular Access·Juan C DuqueRoberto I Vazquez-Padron
Mar 17, 2018·American Journal of Respiratory and Critical Care Medicine·Yves LacasseJerry A Krishnan
Apr 2, 2020·Journal of the Royal Society, Interface·John TarbellColin Caro
Apr 11, 2017·International Journal of Molecular Medicine·Zhui LiXuehu Wang
Jul 12, 2020·European Journal of Clinical Investigation·Eray ErogluOktay Oymak
Jun 3, 2021·International Journal of Molecular Sciences·Yu-Chung ShihDer-Cherng Tarng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Auditory Perception

Auditory perception is the ability to receive and interpret information attained by the ears. Here is the latest research on factors and underlying mechanisms that influence auditory perception.