Supplementation of organ culture medium with dextran is not required in pre-stripped human donor tissue for DMEK surgery

Cell and Tissue Banking
Sabine SallaJohannes Menzel-Severing

Abstract

To assess corneal endothelial cell (CEC) quantity and quality in eye-bank prepared lamellar grafts for Descemet Membrane Endothelial Keratoplasty (DMEK) from organ cultured donor corneas that have not undergone de-swelling by media supplementation with dextran. Prior to graft preparation, corneas that had not undergone de-swelling (n = 30) were placed into fresh storage medium without dextran (KMI). Corneas in the control group (n = 30) were placed in dehydration medium containing 5% dextran (KMII). Subtotal stripping of Descemet's membrane (DM) was performed manually. Following graft preparation, 10 corneas of each group were cultured further in their respective medium for 24 h, 72 h, or 120 h, respectively. Before and after DM stripping, as well as at the end of culture, CEC numbers were obtained. At the end of culture, CEC morphology was graded using a scoring system and CEC metabolism was assessed by detection of adenosine triphosphate. At 24 h after DM stripping, mean CEC counts (in cells/mm2) were 2204 in corneas stored in KMII, and 2391 in corneas stored in KMI (p = 0.003). This corresponds to a mean relative CEC loss of 12.4% with dextran versus 9.7% without dextran (p = 0.04). At 72 h, CEC counts were 1946 in KMII, and...Continue Reading

References

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Citations

Jul 8, 2020·Scientific Reports·Takahiko HayashiClaus Cursiefen
Dec 19, 2020·International Ophthalmology Clinics·David A RamirezChristopher S Sales
Jun 23, 2021·Klinische Monatsblätter für Augenheilkunde·Sabine SallaPeter Walter

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Methods Mentioned

BETA
Assay
Protein Assay

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