Supplementation of scopoletin improves insulin sensitivity by attenuating the derangements of insulin signaling through AMPK

Molecular and Cellular Biochemistry
Kalaivanan KalpanaCarani Venkatraman Anuradha

Abstract

Scopoletin (SPL), a phenolic coumarin, is reported to regulate glucose metabolism. This study is initiated to substantiate the action of SPL on the regulation of insulin signaling in insulin resistant RIN5f cells and high fat, high fructose diet (HFFD)-fed rat model. Adult male Sprague Dawley rats were fed HFFD for 45 days to induce type 2 diabetes and then treated or untreated with SPL for the next 45 days. The levels of glucose, insulin, lipid profile, oxidative stress markers along with insulin signaling and AMPK protein expressions were examined at the end of 90 days. SPL lowered the levels of plasma glucose, insulin, and lipids which were increased in HFFD-fed rats. HFFD intake suppressed the activities of antioxidant enzymes such as superoxide dismutase, catalase, and glutathione peroxidase; however, they were reversed by SPL supplementation, which reduced TBARS, lipid hydroperoxide, and protein carbonyl levels both in plasma and pancreas. SPL supplementation significantly activated insulin receptor substrate 1 (IRS1), phosphatidyl inositol 3-kinase (PI3K), and protein kinase B (Akt) phosphorylation which was suppressed in HFFD rats due to lipotoxicity. Moreover, SPL significantly activated AMPK and enhanced the associati...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·R L LevineE R Stadtman
Oct 17, 1968·European Journal of Biochemistry·W G Niehaus, B Samuelsson
Jun 1, 1972·Analytical Biochemistry·A K Sinha
Feb 9, 1973·Science·J T RotruckW G Hoekstra
Jan 7, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·R N JohnsonJ R Baker
Jul 21, 2000·The Journal of Clinical Endocrinology and Metabolism·A KatzM J Quon
Jul 4, 2002·The Journal of Biological Chemistry·Ann-Kristin Ruud LarsenPer O Seglen
Aug 14, 2003·Phytotherapy Research : PTR·Chin-Ying ShawYing-Chieh Tsai
Apr 14, 2005·Journal of Agricultural and Food Chemistry·María C CarpinellaSara M Palacios
Jul 28, 2006·American Journal of Physiology. Cell Physiology·Jeong-Sun JuJonathan S Fisher
May 18, 2007·Molecular and Cellular Endocrinology·Sung-E ChoiYup Kang
Sep 18, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·D G Hardie
Jul 9, 2009·Physiological Reviews·Gregory R Steinberg, Bruce E Kemp
Jul 27, 2010·European Journal of Pharmacology·Sriramajayam KannappanCarani Venkatraman Anuradha
Aug 24, 2010·Lipids in Health and Disease·Saf-ur Rehman MandukhailAnwarul-Hassan Gilani
Oct 5, 2010·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·W Y ZhangC-S Myung
Oct 7, 2010·Journal of Diabetes·Sumiyabanu Mohamed SalihAnuradha Carani Venkatraman
Oct 21, 2010·The Mount Sinai Journal of Medicine, New York·Emily J GallagherEddy Karnieli
Jul 20, 2011·Nature Chemical Biology·David CarlingSteven J Gamblin
Sep 20, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Tien-Ning ChangGuan-Jhong Huang
Feb 9, 2013·Bioscience, Biotechnology, and Biochemistry·Yonghae SonMyeong-Ho Jung
Sep 13, 2013·Journal of Cell Communication and Signaling·Baskaran YogalakshmiCarani Venkatraman Anuradha
Feb 4, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Eun-Jung ParkMyung-Haing Cho
Mar 18, 2015·Molecular and Cellular Endocrinology·Ik-Rak JungYup Kang
Jul 1, 2015·Journal of Basic and Clinical Physiology and Pharmacology·Mutlur Krishnamoorthy Radika, Carani Venkatraman Anuradha
Feb 27, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jagan KalivarathanAnuradha Carani Venkatraman

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Citations

Mar 5, 2020·Proteomics·Venkateswaran NatarajanKarthik Mallilankaraman
May 8, 2020·Oxidative Medicine and Cellular Longevity·Emad H M HassaneinAyman M Mahmoud

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Methods Mentioned

BETA
ELISA
electrophoresis
fluorescence imaging

Software Mentioned

Image J
SPSS
Prism

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