Supplementation of the dilution medium after thawing with reduced glutathione improves function and the in vitro fertilizing ability of frozen-thawed bull spermatozoa

International Journal of Andrology
Joaquin GadeaJ C Gardon

Abstract

In this study, we evaluated the effects of glutathione (l-gamma-glutamyl-l-cysteinylglycine; GSH) supplementation of the thawing extender on bull semen parameters to compensate for the decrease in GSH content observed during sperm freezing. To address these questions fully, we used a set of functional sperm tests. These included tests of sperm motility assayed by computer-assisted semen analysis, membrane lipid packing disorder, spontaneous acrosome reaction, free radical production [reactive oxygen species (ROS) generation], sperm chromatin condensation, DNA fragmentation by terminal deoxynucleotidyltransferase-mediated dUTP nick-end labelling and acridine orange staining measured by flow cytometry. Finally, the in vitro penetrability of in vitro matured oocytes and the in vitro production of embryos were evaluated. The main findings emerging from this study were that addition of GSH to the thawing medium resulted in: (i) a higher number of non-capacitated viable spermatozoa; (ii) a reduction in ROS generation; (iii) lower chromatin condensation; (iv) lower DNA fragmentation; (v) higher oocyte penetration rate in vitro and (vi) higher in vitro embryo production compared with control group. Nevertheless, GSH had no significant ...Continue Reading

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Citations

Feb 23, 2010·Asian Journal of Andrology·Juan Carlos Martinez-SotoJoaquín Gadea
Sep 3, 2011·Cryobiology·Juan Carlos Martínez-SotoJoaquín Gadea
May 8, 2018·The Journal of Reproduction and Development·Toshinori OikawaTakashi Numabe
Aug 7, 2021·Antioxidants·Eliana Pintus, José Luis Ros-Santaella

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