Supplementation with α-ketoglutarate to a low-protein diet enhances amino acid synthesis in tissues and improves protein metabolism in the skeletal muscle of growing pigs

Amino Acids
Jiashun ChenKang Yao

Abstract

α-Ketoglutarate (AKG) is a crucial intermediate in the tricarboxylic acid (TCA) cycle and can be used for the production of ATP and amino acids in animal tissues. However, the effect of AKG on the expression patterns of genes involved in muscle protein metabolism is largely unknown, and the underlying mechanism remains to be elucidated. Therefore, we used young pigs to investigate the effects of a low crude protein (CP) diet and a low CP diet supplemented with AKG on protein accretion in their skeletal muscle. A total of 27 growing pigs with an initial body weight of 11.96 ± 0.18 kg were assigned randomly to one of the three diets: control (normal recommended 20% CP, NP), low CP (17% CP, LP), or low CP supplemented with 1% AKG (ALP). The pigs were fed their respective diets for 35 days. Free amino acid (AA) profile and hormone levels in the serum, and the expression of genes implicated in protein metabolism in skeletal muscle were examined. Results showed that compared with the control group or LP group, low-protein diets supplemented with AKG enhanced serum and intramuscular free AA concentrations, the mRNA abundances of AA transporters, and serum concentrations of insulin-like growth factor-1 (IGF-1), activated the mammalian ...Continue Reading

References

Mar 24, 1990·Lancet·J WernermanE Vinnars
Jan 1, 1991·Annual Review of Physiology·J R FloriniK A Magri
Jun 1, 1988·The American Journal of Physiology·L W Johnson, C H Smith
Jun 12, 1999·European Journal of Clinical Nutrition·P J GarlickC S Patlak
Aug 26, 1999·International Journal of Colorectal Disease·J Wernerman, F Hammarqvist
Dec 1, 1999·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·M Jeevanandam, S R Petersen
Feb 13, 2001·American Journal of Physiology. Endocrinology and Metabolism·W E TaylorN Gonzalez-Cadavid
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 24, 2004·Journal of Animal Physiology and Animal Nutrition·N B KristensenS G Pierzynowski
Dec 9, 2004·Current Opinion in Clinical Nutrition and Metabolic Care·Erica P A RuttenNicolaas E P Deutz
Jul 5, 2006·Genes & Development·Xiaozhong Shi, Daniel J Garry
Jun 1, 1991·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·M JeevanandamW R Schiller
Oct 24, 2006·The Journal of Nutrition·Barry D LambertDouglas G Burrin
Jan 16, 2008·Physiological Reviews·Stefan Bröer
Jun 17, 2008·Physiology·Marco Sandri
Oct 22, 2008·Scandinavian Journal of Clinical and Laboratory Investigation·Radoslaw P RadzkiStefan G Pierzynowski
Nov 28, 2008·Journal of Applied Physiology·Mitsunori Miyazaki, Karyn A Esser
Dec 6, 2008·Current Opinion in Clinical Nutrition and Metabolic Care·Teresa A Davis, Marta L Fiorotto
Mar 27, 2009·Carcinogenesis·Daniel A TennantEyal Gottlieb
Mar 23, 2010·American Journal of Physiology. Endocrinology and Metabolism·Micah J DrummondBlake B Rasmussen
Jan 5, 2011·Frontiers in Bioscience (Landmark Edition)·Yongqing HouGuoyao Wu
Sep 1, 2004·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Lynn Taylor, Norman P Curthoys
Sep 29, 2011·The Journal of Physiology·Craig A GoodmanTroy A Hornberger
Jun 8, 2012·Journal of Cachexia, Sarcopenia and Muscle·Alessandro FanzaniWim Martinet
Jul 4, 2012·Molecular Cell·Raúl V DuránMichael N Hall

❮ Previous
Next ❯

Citations

Jan 18, 2020·Animals : an Open Access Journal From MDPI·Benedetta TugnoliEster Grilli
Feb 16, 2020·Animals : an Open Access Journal From MDPI·Simeng LiaoYulong Tang
Jun 27, 2020·Oxidative Medicine and Cellular Longevity·Simeng LiaoYulong Tang
Oct 1, 2020·Animals : an Open Access Journal From MDPI·Giulia Ferronato, Aldo Prandini
Dec 20, 2020·Ageing Research Reviews·Maria M Bayliak, Volodymyr I Lushchak
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yantong LiDonghai Lin

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
ion-exchange chromatography
electrophoresis
PCR
transgenic

Software Mentioned

SPSS
Alpha Imager

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.