Supplements to in vitro maturation media affect the production of bovine blastocysts and their apoptotic index but not the proportions of matured and apoptotic oocytes

Animal Reproduction Science
E WarzychD Lechniak

Abstract

The objective of this study was to compare the effect of different supplements to the basic IVM medium (TCM199) on the efficiency of cattle oocyte maturation and blastocyst production, and the incidence of apoptosis in both oocytes and blastocysts. Two protein supplements (FBS and fafBSA) and a macromolecule (PVP40) were compared in a 3 treatmentsx9 replicates design. Cumulus-oocyte complexes (COCs) aspirated from slaughterhouse ovaries were matured for 24h in TCM199 medium supplemented with 10% FBS, 6% fafBSA or 4% PVP40 (50-70 COCs in each treatment/replicate), then inseminated and cultured in vitro for 8 days. Immature and mature oocytes as well as Day 8 blastocysts were subjected to TUNEL analysis. Cleavage rate was monitored on Day 2 post-insemination (pi), whereas blastocyst yield on Day 8 pi. The composition of maturation media did not affect zygotic cleavage rate on Day 2 (on average 71.0%), however the blastocyst rate on Day 8 pi was significantly lower (P<0.001) for embryos derived from oocytes matured with PVP40 (16.0%) than for those matured with FBS (22.4%) or fafBSA (22.1%). The rate of TUNEL positive oocytes differed significantly between immature (1.4%) and mature (11.2%) oocytes (P<0.01). Supplements to maturat...Continue Reading

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Citations

Feb 2, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Helmut TornerDawit Tesfaye
Jan 20, 2009·Theriogenology·M G Katz-JaffeD K Gardner
Jan 12, 2010·Reproduction in Domestic Animals = Zuchthygiene·G LopesA Rocha
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Jun 11, 2014·Zygote : the Biology of Gametes and Early Embryos·Gustavo Bruno MotaAlzira Amélia Martins Rosa E Silva
Dec 1, 2011·Reproduction, Fertility, and Development·Shan LiuAvner Hershlag
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May 1, 2018·Animal Biotechnology·Jolanta OpielaJoanna Romanek
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Dec 24, 2018·International Journal of Molecular Sciences·Krishna Chaitanya PavaniBart Leemans
Jan 5, 2021·Frontiers in Cell and Developmental Biology·Priscila Ramos-IbeasEnrique Gómez

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis