Supported Lipid Bilayer Technology for the Study of Cellular Interfaces

Current Protocols in Cell Biology
Travis J CritesRajat Varma

Abstract

Glass-supported lipid bilayers presenting freely diffusing proteins have served as a powerful tool for studying cell-cell interfaces, in particular, T cell-antigen presenting cell (APC) interactions, using optical microscopy. Here we expand upon existing protocols and describe the preparation of liposomes by an extrusion method, and describe how this system can be used to study immune synapse formation by Jurkat cells. We also present a method for forming such lipid bilayers on silica beads for the study of signaling responses by population methods, such as western blotting, flow cytometry, and gene-expression analysis. Finally, we describe how to design and prepare transmembrane-anchored protein-laden liposomes, following expression in suspension CHO (CHOs) cells, a mammalian expression system alternative to insect and bacterial cell lines, which do not produce mammalian glycosylation patterns. Such transmembrane-anchored proteins may have many novel applications in cell biology and immunology.

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Citations

Nov 2, 2016·The Journal of Cell Biology·Sricharan MurugesanJohn A Hammer
Nov 23, 2019·Medical & Biological Engineering & Computing·Sara AbbaspourMax Ortiz-Catalan
Sep 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ashley R AmbroseDaniel M Davis
Jan 20, 2021·ACS Biomaterials Science & Engineering·Johana UribeSusan Daniel
Feb 7, 2020·The Journal of Physical Chemistry. B·Daniel BeckersErdinc Sezgin
Nov 23, 2021·ACS Biomaterials Science & Engineering·Johana UribeSusan Daniel

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