Suppressing Dazl modulates tumorigenicity and stemness in human glioblastoma cells

BMC Cancer
Fengyu ZhangYuan Lu

Abstract

Glioblastoma is devastating cancer with a high frequency of occurrence and poor survival rate and it is urgent to discover novel glioblastoma-specific antigens for the therapy. Cancer-germline genes are known to be related to the formation and progression of several cancer types by promoting tumor transformation. Dazl is one such germline gene and is up-regulated in a few germ cell cancers. In this study, we analyzed the expression of Dazl in human glioblastoma tissues and cells, and investigated its significance in proliferation, migration, invasion and chemoresistance of the glioblastoma cell lines. We evaluated the expression of Dazl in different pathologic grades of glioblastoma tissues by immunohistochemistry. We assessed the expression of Dazl in glioblastoma cells and normal human astrocytes (NHA) cells by western blotting and RT-qPCR. Then we generated Dazl knockout glioblastoma cell lines using the CRISPR/Cas9 gene-editing technology to explore the cellular function of Dazl. We detected the proliferation and germline traits via CCK-8 assays and alkaline phosphatase staining, respectively. Boyden chamber assays were performed to measure glioblastoma cell migration and invasion. Crystal violet staining was used to determ...Continue Reading

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Citations

May 4, 2021·Seminars in Cancer Biology·Chunfang LiuHui-Kuan Lin
Sep 18, 2021·Scientific Reports·Stefanie BinderMaik Friedrich

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Methods Mentioned

BETA
surgical resection
Transfection
electrophoresis
xenograft

Software Mentioned

GraphPad Prism
ARRIVE
Image J

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