Suppression of adverse angiogenesis in an albumin-based hydrogel for articular cartilage and intervertebral disc regeneration

European Cells & Materials
B ScholzBurkhard Schlosshauer

Abstract

An injectable polyethylene glycol-crosslinked albumin gel (AG) supplemented with hyaluronic acid as a matrix for autologous chondrocyte implantation was evaluated with regard to its impact on angiogenesis. Healthy articular cartilage and intervertebral discs (IVD) are devoid of blood vessels, whereas pathological blood vessel formation augments degeneration of both theses tissues. In contrast to human endothelial cells, primary human articular chondrocytes encapsulated in the AG retained their viability. Endothelial cells did not adhere to the gel surface to a significant extent nor did they proliferate in vitro. The AG did not release any diffusible toxic components. Contrary to Matrigel employed as positive control, the AG prevented endothelial chemoinvasion in Transwell filter assays even in the presence of a chemotactic gradient of vascular endothelial growth factor. In ovo, the AG exhibited a barrier function for blood vessels of the chick chorioallantoic membrane. Subcutaneous implantation of human IVD chondrocytes enclosed in the albumin gel into immunodeficient mice revealed a complete lack of angiogenesis inside the gel after two weeks. At the same time, the IVD chondrocytes within the gel remained vital and displayed ...Continue Reading

Citations

May 8, 2014·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·G W OmlorW Richter
Jul 1, 2012·Cartilage·Daniela Filipa Duarte CamposHorst Fischer
Dec 9, 2017·Hip International : the Journal of Clinical and Experimental Research on Hip Pathology and Therapy·Steffen ThierStefan Fickert
Jan 18, 2020·International Journal of Molecular Sciences·Aziz EftekhariMagali Cucchiarini
Apr 12, 2012·BMC Musculoskeletal Disorders·Karin BenzJuergen A Mollenhauer
Jul 25, 2013·Archives of Orthopaedic and Trauma Surgery·F MedvedH E Schaller
Sep 15, 2019·Biotechnology and Bioengineering·John OngAthina E Markaki
Oct 12, 2012·Journal of Materials Science. Materials in Medicine·Cecilia Persson, Svante Berg
Jul 31, 2012·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Karin BenzJürgen A Mollenhauer
Feb 8, 2019·Current Pharmaceutical Biotechnology·Marta A SzychlinskaGiuseppe Musumeci
Dec 20, 2013·Journal of the Royal Society, Interface·S I CorreiaR L Reis
Dec 14, 2011·Journal of Tissue Engineering and Regenerative Medicine·Antje MietschAnita Ignatius
Feb 25, 2017·Biomaterials Science·Yon Jin ChuahDong-An Wang
Apr 18, 2019·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Henriette BretschneiderStefan Fickert
Jan 16, 2017·Acta Biomaterialia·Jingzhou YangAli Khademhosseini
Dec 12, 2016·ACS Biomaterials Science & Engineering·Nicolas BroguiereMarcy Zenobi-Wong
Oct 28, 2021·Expert Reviews in Molecular Medicine·Martin Philipp DieterlePascal Tomakidi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.