Abstract
Short 21-mer double-stranded/small-interfering RNAs (ds/siRNAs) were designed to target bcr-abl mRNA in chronic myelogenous leukemia. The ds/siRNAs were transfected into bcr-abl-positive K-562 (derived from blast crisis chronic myelogenous leukemia), using lipofectamine. Penetrating of ds/siRNAs into the cells was detected by fluorescent confocal microscopy, using fluorescein-labeled ds/siRNAs. The cells were treated with mix of three siRNA sequences (3 x 60 nM) during 6 days with three repetitive transfections. The siRNA-treatment was accompanied with significant reduction of bcr-abl mRNA, p210, protein tyrosine kinase activity and cell proliferation index. Treatment of cells with Glivec (during 8 days with four repetitive doses, 180 nM single dose) resulted in analogous reduction of cell proliferation activity, stronger suppression of protein tyrosine kinase activity, and very low reduction of p210. siRNA-mix and Glivec did not affect significantly the viability of normal lymphocytes. Microarray analysis of siRNA- and Glivec-treated K-562 cells demonstrated that both pathways of bcr-abl suppression were accompanied with overexpression and suppression of many different oncogenes, apoptotic/antiapoptotic and cell proliferation ...Continue Reading
References
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·M K MontgomeryA Fire
May 14, 1999·Current Opinion in Plant Biology·D C Baulcombe
Aug 10, 2000·Nature Biotechnology·J R Kennerdell, R W Carthew
May 25, 2001·Nature·S M ElbashirT Tuschl
Jan 31, 2002·Annual Review of Medicine·Michael E O'DwyerBrian J Druker
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Patrick J PaddisonGregory J Hannon
Feb 21, 2002·Lancet·Nikolas von BubnoffJustus Duyster
Feb 28, 2002·Blood·Wolf-K HofmannH Phillip Koeffler
Aug 13, 2002·Oncogene·Monika WildaArndt Borkhardt
Aug 23, 2002·Pharmacology & Therapeutics·Doriano FabbroPaul W Manley
Oct 24, 2002·Blood·Michaela ScherrMatthias Eder
Dec 12, 2002·Oncogene·Richard A Van Etten
Feb 7, 2003·Current Medicinal Chemistry·Michaela ScherrMatthias Eder
Apr 16, 2003·Cell Cycle·Barbara ArdeltZbigniew Darzynkiewicz
May 17, 2003·Blood·Lara WohlboldWalter E Aulitzky
May 30, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Laura R Saunders, Glen N Barber
Jun 12, 2003·Annals of the New York Academy of Sciences·Tim H BrummendorfAndreas Hochhaus
Oct 31, 2003·Lancet·Nathan R Wall, Yang Shi
Nov 6, 2003·Biochemical Pharmacology·Rumiana BakalovaYoshinobu Baba
Apr 20, 2004·FEBS Letters·Rumiana BakalovaYoshinobu Baba
Citations
Apr 5, 2005·Functional & Integrative Genomics·Hailing LiuGeoffrey Childs
Mar 21, 2006·Journal of Biomedical Science·Benjamin E BakerAlbert T Ichiki
Apr 10, 2009·Leukemia·S KobayashiK Motoyoshi
Dec 17, 2009·The Journal of Biological Chemistry·Hongfeng YuanWenYong Chen
Sep 27, 2007·Oligonucleotides·Takanori KuboRumiana Bakalova
Feb 24, 2006·Acta Pharmacologica Sinica·Maria ThomasOlaf Heidenreich
Dec 18, 2013·Pharmacology & Therapeutics·Jianliang Zhang, Steven N Hochwald
Mar 13, 2013·Biomaterials·Jin Sook SuhYoon Jeong Park
Apr 21, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yamini ArthanariConstantinos Demonacos
May 5, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Toshihito TsutsumiHidetoshi Arima
Jan 9, 2008·Genes, Chromosomes & Cancer·Petra HåkanssonThoas Fioretos
May 13, 2015·Molecular Therapy. Nucleic Acids·Breanne LandryHasan Uludag
Jun 4, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Juliana Valencia-SernaHasan Uludağ
Mar 25, 2006·Future Oncology·Hermann Lage
Mar 4, 2008·Molecular Cancer Research : MCR·Konrad HuppiNatasha J Caplen
Jan 6, 2010·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Mikhail DoubrovinSteven M Larson
Aug 11, 2005·Journal of the American Chemical Society·Rumiana BakalovaYoshinobu Baba