PMID: 8611635Feb 2, 1996Paper

Suppression of Ca2+ oscillations in glucagon-producing alpha 2-cells by insulin/glucose and amino acids

Biochimica Et Biophysica Acta
A BertsBo Hellman

Abstract

The cytoplasmic Ca2+ concentration ([Ca2+]i) was continuously monitored in single glucagon-producing alpha 2-cells isolated from the mouse pancreas and later identified by immunostaining. Up to 60% of the alpha 2-cells exhibited spontaneous [Ca2+]i oscillations (frequency 0.1-0.3/min) in a medium containing 3 mM glucose. In originating from a basal level of 60-100 nM, reaching peak values of 300-400 nM and promptly disappearing after blocking voltage-dependent Ca2+ channels with methoxyverapamil, the oscillations resembled those in insulin-releasing beta-cells stimulated by glucose. The oscillatory activity was suppressed when combining elevation of glucose to 20 mM with the addition of 2-2000 ng/ml insulin. Whereas 10 mM of L-arginine or l-glycine transformed the oscillations into sustained elevation of [Ca2+]i, there was no response to 1 mM tolbutamide or 0.1-1 mM gamma-aminobutyric acid. The observations that alpha 2-cells differ from islet cells secreting insulin and somatostatin in responding to adrenaline with mobilisation of intracellular calcium can be used for their rapid identification. It is suggested that the oscillations reflect periodic entry of Ca2+ due to variations of the membrane potential.

References

Dec 11, 1992·Biochimica Et Biophysica Acta·B HellmanA Berts
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Citations

Oct 1, 2006·Journal of Biological Physics·Paul M Diderichsen, Sven O Göpel
May 22, 2002·Life Sciences·Fumi SuginoKoichi Nakayama
Mar 14, 2012·PloS One·Virginia González-VélezIván Quesada
Dec 26, 2013·Diabetes Research and Clinical Practice·Erik Gylfe, Patrick Gilon
Apr 6, 2016·Upsala Journal of Medical Sciences·Erik Gylfe
Oct 24, 1998·General and Comparative Endocrinology·L RuffierN Rideau
Mar 17, 2010·The Journal of Biological Chemistry·Sylvain J Le Marchand, David W Piston
Jun 19, 2019·Physical Biology·Taegeun Song, Junghyo Jo
Jan 31, 2007·Endocrine Reviews·Jesper GromadaClaes B Wollheim
Feb 4, 2016·American Journal of Physiology. Endocrinology and Metabolism·Margaret WattsArthur Sherman
Aug 7, 2010·Hormones : International Journal of Endocrinology and Metabolism·Paloma Alonso-MagdalenaAngel Nadal
Oct 13, 2018·Nature Reviews. Endocrinology·Jesper GromadaGuy A Rutter
Apr 1, 1997·Metabolism: Clinical and Experimental·A BertsB Hellman

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