PMID: 46635Jan 1, 1975

Suppression of connective tissue impregnation in a silver technique for demonstrating nerve fibers

Stain Technology
P R Herdman, J J Taylor

Abstract

A tissue pretreatment is introduced which effectively suppresses the silver impregnation of connective tissue and nonspecific background elements in peripheral nerve. The result is a selective impregnation of nerve fibers. The procedure utilizes fresh frozen sections and can be used with the Holmes (1947) or Bodian (1936) techniques. Fresh frozen sections are cut at 10 microns, mounted on slides and air dried for 5 minutes. They are fixed for 30 minutes in formol-sublimate (10% formalin saturated with mercuric chloride) and then placed into 0.5% iodine in 70% alcohol for 5 minutes followed by bleaching in 2.5% sodium thiosulfate for 2 minutes. After washing in running tap water for 10 minutes and a brief rinse in distilled water, impregnation is accomplished by the Holmes (1947) or Bodian (1936) procedure beginning with the step containing the aqueous silver solution. The results show an absence of impregnation of connective tissue and nonspecific background. The technique is simple, rapid, and, by utilizing fresh frozen sections, can be used for other histological and histochemical purposes. Several experiments were done to determine the causes of the connective tissue and background suppression. The air drying step was omitte...Continue Reading

References

Sep 1, 1976·Stain Technology·B E HerrR C Griggs
Mar 1, 1988·Stain Technology·R S Liem, J Douwe van Willigen
Apr 13, 1975·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·A D Blest

Citations

Apr 1, 1972·Journal of General Microbiology·M Herdman, N G Carr

Related Concepts

Electron Microscopy
Perineurium
Staining and Labeling
Freeze Drying
Myelinated Nerve Fiber
Cytochemistry
Silver
Connective Tissue

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