Suppression of cyclic guanosine monophosphate formation in rat cerebellar slices by propofol, ketamine and midazolam

Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie
I MiyawakiK Mori

Abstract

The nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) system is involved in glutamatergic neurotransmission. The current study determined the effects of propofol, ketamine and midazolam on rat cerebellar cGMP formation, attempting to clarify whether the effect was due to suppression of NO-cGMP system or to direct interaction with glutamatergic receptors. Cerebellar slices, obtained from six- to eight-day-old Wistar rats, were pretreated with propofol (10 microM-1 mM), ketamine (10-100 microM) or midazolam (1-100 microM) for 30 min. and then stimulated with L-glutamate (3 mM), N-methyl-D-aspartate (NMDA, 0.1 mM), kainate (0.1 mM) or sodium nitroprusside (SNP, 0.3 mM) (n = 5-11 for each group). The levels of cGMP were determined by radioimmunoassay. None of the anaesthetics studied altered cGMP levels when no stimulant was given. Propofol (10 microM-1 mM) suppressed L-glutamate-, NMDA-, kainate- and SNP stimulated cGMP formation in a concentration-dependent manner, the sensitivity to propofol was in the order of NMDA > kainate > L-glutamate. SNP. Ketamine (10-100 microM) suppressed L-glutamate- and NMDA-stimulated cGMP formation, but did not suppress kainate- or SNP-stimulated cGMP formation. Midazolam (10-100 microM) did n...Continue Reading

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Citations

Sep 19, 2012·Journal of Clinical Anesthesia·Robert H ThieleDanja S Groves
Mar 8, 2016·Life Sciences·Wenguo FanHongwen He
Feb 28, 2009·European Journal of Pharmacology·Ioanna VasileiouLila Papadimitriou
May 9, 2018·Clinical Psychopharmacology and Neuroscience : the Official Scientific Journal of the Korean College of Neuropsychopharmacology·Kwan Young LeeDong Kwan Kim
Dec 13, 2007·Anesthesiology·Noboru TodaYoshio Hatano
Sep 28, 2002·Anesthesia and Analgesia·Tadanobu YasudaAkitomo Matsuki

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