PMID: 9660179Jul 11, 1998Paper

Suppression of decorin expression and partial induction of anchorage-independent growth by the v-src oncogene in human fibroblasts

European Journal of Biochemistry
E Kolettas, R F Rosenberger

Abstract

To determine its role in cell transformations, the v-src oncogene was introduced into the human foetal diploid fibroblasts MRC-5 and into MRC-SV1, a simian virus 40 (SV40)-transformed cell line derived from them. Infected cells were found to contain stably integrated intact proviruses, as determined by Southern blot analysis. Although highly expressed, v-src did not change the morphology or growth patterns of MRC-5 cells and failed to induce foci or alter their saturation densities. However, overexpression of v-src reduced the plating efficiencies of MRC-5 and induced anchorage-independent growth in a low but significant number of cells. Northern blot analysis showed that v-src selectively abolished the expression of decorin, a small dermatan/chondroitin sulphate proteoglycan that interacts with extracellular-matrix components and modulates collagen-fibril formation and the activity of transforming growth factor (TGF) beta1. Addition of herbimycin A, a potent pp60src tyrosine-kinase inhibitor, resulted in the reexpression of decorin in MRC-5 carrying v-src. There were no changes in the expressions of fibronectin, procollagen type I, or tissue plasminogen activator, an activator of extracellular-matrix-degrading enzymes. Moreove...Continue Reading

Citations

Aug 15, 2002·Experimental Gerontology·E S GonosD H Vynios
Sep 14, 2011·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Connie S ChamberlainRay Vanderby
Jul 1, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Kien T TranAlan Wells
Mar 10, 2000·Biochemical and Biophysical Research Communications·D CoppockD A Cina-Poppe
Oct 4, 2011·Cellular and Molecular Life Sciences : CMLS·Elena GarusiRoberto Perris
Apr 12, 2005·Cancer Science·Hiroshi SatoHisashi Miyamori
May 30, 2006·Oral Oncology·Mei-Huei LinYoung-Chau Liu
Oct 18, 2001·Clinical Orthopaedics and Related Research·W H AkesonA Giurea

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