PMID: 7028859Oct 1, 1981Paper

Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
G S Wood, R Warnke

Abstract

As biotin-avidin systems continue to be developed for applications involving single cells, cell suspensions, and especially tissue sections, the need arises for a method of blocking endogenous avidin-binding activity. One such method is described and its proposed mechanism is discussed. Utilizing this method, endogenous avidin-binding activity was detected and suppressed in selected human and murine tissues, thus facilitating the interpretation of specific immunohistochemical staining utilizing hybridoma monoclonal antibodies in a biotin-avidin-horseradish peroxidase detection system.

Citations

Jul 1, 1997·The Journal of Pathology·A M McNicol, M A Farquharson
Apr 10, 1997·International Journal of Cancer. Journal International Du Cancer·E JägerA Knuth
Mar 1, 1992·Genes, Chromosomes & Cancer·C A GriffinR Mann
Jun 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K TsuneyamaY Nakanuma
Sep 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K TsuneyamaY Nakanuma
Jan 1, 1983·Archives of Dermatological Research·E BertiR Caputo
Mar 1, 1983·The Histochemical Journal·F T Bosman
Jun 1, 1993·Digestive Diseases and Sciences·B ShneiderP Buckley
Jan 1, 1993·Virchows Archiv. A, Pathological Anatomy and Histopathology·A MaedaT Kurata
May 1, 1987·The Histochemical Journal·C J JonesR W Stoddart
Feb 1, 1988·The Histochemical Journal·G CattorettiF Rilke
Oct 1, 1996·Diseases of the Colon and Rectum·S MooteriT Saclarides
May 1, 1994·Digestive Diseases and Sciences·D BaniT B Sacchi
Jun 17, 2008·Analytical and Bioanalytical Chemistry·Olivier LaczkaFrancesc Xavier Muñoz
Jan 14, 2005·Virchows Archiv : an International Journal of Pathology·Yasuhiko KimuraShigeo Yokoyama
May 1, 1994·Clinica Chimica Acta; International Journal of Clinical Chemistry·M MusianiM La Placa
Nov 15, 1996·Clinica Chimica Acta; International Journal of Clinical Chemistry·J Hymes, B Wolf
Feb 24, 1984·Journal of Immunological Methods·W S WalkerS E Yen
Feb 26, 1987·Journal of Immunological Methods·R T KenneyD M Scollard
Apr 1, 1993·Comparative Immunology, Microbiology and Infectious Diseases·M Olovsson, A Larsson
Jan 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·G GriffonP Bey

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