PMID: 45403Jun 1, 1979

Suppression of graft-versus-host reaction by preincubation of the graft with an antitumor protein, neocarzinostatin

Immunopharmacology
A YamashitaH Maeda

Abstract

The immunosuppressive activity of neocarzinostatin, an antitumor antibiotic with a high molecular weight, was demonstrated, as measured by the local and systemic graft-versus-host reaction in rats. The preincubation of parental strain lymphocytes with doses more than 1 micrograms/ml of neocarzinostatin for at least 10 min at 37 degrees C resulted in the marked suppression of the popliteal lymph node enlargement, when injected subcutaneously into the hind footpads of F1 hybrid rats. The suppressive effect was temperature-dependent and irreversible, because the neocarzinostatin (1 microgram/ml) pretreatment of parental lymphocytes at 4 degrees C was not effective in the suppressive activity, and the reincubation of cells after the removal of the drug from the culture did not result in demonstrable changes in the degree of suppression. The neocarzinostatin pretreatment does not result in visible changes in the oxygen consumption rate and viability of cells in vitro or in the distribution pattern within a regional lymph node, indicating that treated cells may retain the same viability and migration capacity in vivo as do untreated cells. Furthermore, the drug pretreatment resulted in the complete prevention of runting syndrome, whe...Continue Reading

References

May 1, 1976·Cellular Immunology·J J LipskyP S Lietman
Apr 1, 1973·Proceedings of the Society for Experimental Biology and Medicine·K F SwingleP M Valle
Dec 1, 1973·The Australian Journal of Experimental Biology and Medical Science·P McCullagh
Oct 1, 1973·European Journal of Immunology·I FlorentinG Mathé
Oct 1, 1974·Archives of Biochemistry and Biophysics·H MaedaJ Meienhofer
Nov 24, 1972·Science·J MeienhoferK Kuromizu
Jan 14, 1964·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J L GOWANS, E J KNIGHT

Related Concepts

Metazoa
Antibiotics, Cytotoxic
Dose-Response Relationship, Drug
Graft-vs-Host Disease
Graft Vs Host Reaction
Immunosuppressive Agents
Lymphoid Cells
Zinostatin
August Rats
Lymphocyte Transfusion

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