Suppression of hepatocellular carcinoma growth via oral immune regulation towards tumor-associated antigens is associated with increased NKT and CD8+ lymphocytes

Oncology
Oren ShiboletYaron Ilan

Abstract

Oral immune regulation towards viral proteins was previously shown to modulate the anti-HBV immune response. Adoptive transfer of orally immunomodulated lymphocytes suppressed the growth of hepatocellular carcinoma (HCC) expressing HBsAg in athymic mice. NKT lymphocytes play a role in the defense against tumor growth. To evaluate the effect of oral immune regulation towards HCC-associated antigens or HBV proteins on growth of HBsAg-expressing HCC, and to determine the role of NKT lymphocytes in immune modulation. Sublethally irradiated athymic Balb/c mice were injected with 10(7) human hepatoma cells followed 10 days later by transplantation of 2 x 10(6) splenocytes from naive donor mice. Immune modulation was performed via feeding of HCC-extracted proteins or HBV antigens (HBsAg + Pre S1 + Pre S2). The control group was fed with bovine serum albumin (BSA). Mice were followed for survival, tumor volume, and serum alpha-fetoprotein levels. To determine the role of NKT cells in tumor suppression, cytokine expression and FACS analysis for CD4+, CD8+, and NK1.1+ T lymphocyte subsets were performed. Oral immune regulation towards HCC-extracted proteins induced complete tumor suppression in recipient mice. Mortality rates were 0% in ...Continue Reading

Citations

Nov 9, 2005·International Immunopharmacology·Yongyan ChenZhigang Tian
Nov 22, 2012·Journal of Clinical Biochemistry and Nutrition·Yasuhiro TsudaKazuhide Higuchi
Jul 24, 2015·Hepatology International·Yaron Ilan
Oct 16, 2009·Expert Review of Anticancer Therapy·Evangelia M Fatourou, John S Koskinas
Feb 3, 2005·International Journal of Cancer. Journal International Du Cancer·Maya MargalitYaron Ilan
Jun 30, 2009·Human Immunology·Yaron Ilan
Dec 25, 2010·Therapeutic Advances in Gastroenterology·Eran Israeli, Yaron Ilan
Feb 16, 2011·Laboratory Investigation; a Journal of Technical Methods and Pathology·Mengde CaoChen Liu
Jun 24, 2008·Journal of Immunotoxicology·Tomer Adar, Yaron Ilan
May 20, 2015·World Journal of Gastroenterology : WJG·Eran IsraeliYaron Ilan

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