Suppression of human hepatoma (HepG2) cell growth by nuclear factor-kappaB/p65 specific siRNA.

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Wei WuDengbing Yao

Abstract

Nuclear factor-kappaB (NF-κB) is a transcription factor and antagonist of apoptosis during liver regeneration and closely related to the formation and development of hepatocellular carcinoma. In the present study, we investigated the effect of small interference RNA (siRNA)-mediated inhibition of NF-κB on growth of human hepatoma (HepG2) cells. Our data indicated that the expression of NF-κB/p65 mRNA was significantly higher in the HepG2 cells than that in the normal liver (LO2) cells before transfection, and the expression of NF-κB/p65 in the HepG2 cells with NF-κB/p65 siRNA (100 nMol/L) transfection at 72 h was reduced at the levels of mRNA (93%) and protein (62%) using real-time reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, and Western blotting. Interestingly, the apoptosis index of the HepG2 cells increased up to 85%, detected by Annexin V-fluorescein isothiocyanate, suggesting that NF-κB is overexpressed in hepatoma cells and can be inhibited by NF-κB/p65 siRNA through the apoptotic mechanism. Thus, we conclude that NF-κB is a potential molecular target for HCC gene therapy.

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Citations

Aug 3, 2012·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·A DeezagiN Vaseli-Hagh
Nov 30, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Dandan YuDengfu Yao
Mar 19, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Min YaoDengfu Yao
Jun 5, 2014·Nutrition and Cancer·Harshita ChaudharySriram Seshadri
Mar 9, 2019·Metallomics : Integrated Biometal Science·Yunlan LiQingshan Li
Aug 30, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Hanaa Hibishy GaballahSaber Abdelrahman Ismail

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