Suppression of integrin alpha3beta1 in breast cancer cells reduces cyclooxygenase-2 gene expression and inhibits tumorigenesis, invasion, and cross-talk to endothelial cells.

Cancer Research
Kara MitchellC Michael DiPersio

Abstract

Integrin receptors for cell adhesion to extracellular matrix have important roles in promoting tumor growth and progression. Integrin alpha3beta1 is highly expressed in breast cancer cells in which it is thought to promote invasion and metastasis; however, its roles in regulating malignant tumor cell behavior remain unclear. In the current study, we used short-hairpin RNA (shRNA) to show that suppression of alpha3beta1 in a human breast cancer cell line, MDA-MB-231, leads to decreased tumorigenicity, reduced invasiveness, and decreased production of factors that stimulate endothelial cell migration. Real-time PCR revealed that suppression of alpha3beta1 caused a dramatic reduction in expression of the cyclooxygenase-2 (COX-2) gene, which is frequently overexpressed in breast cancers and has been exploited as a therapeutic target. Decreased COX-2 was accompanied by reduced prostaglandin E2 (PGE(2)), a major prostanoid produced downstream of COX-2 and an important effector of COX-2 signaling. shRNA-mediated suppression of COX-2 showed that it has a role in tumor cell invasion and cross-talk to endothelial cells. Furthermore, treatment with PGE(2) restored these functions in alpha3beta1-deficient MDA-MB-231 cells. These findings i...Continue Reading

References

Jan 12, 2000·Oncogene·C S WilliamsR N DuBois
Sep 9, 2000·Current Opinion in Cell Biology·J A Kreidberg
Oct 12, 2000·Nature Cell Biology·G BergersD Hanahan
Mar 30, 2001·The Journal of Biological Chemistry·C H LiuT Hla
Jun 9, 2001·Endocrine-related Cancer·L R HoweA J Dannenberg
Aug 2, 2001·The Journal of Biological Chemistry·F BerditchevskiS J Jenner
Sep 8, 2001·Journal of Mammary Gland Biology and Neoplasia·A M MercurioT Tani
Mar 22, 2002·The Lancet Oncology·A J DannenbergK Subbaramaiah
Apr 17, 2002·The Journal of Clinical Investigation·Cord BrakebuschReinhard Fässler
May 14, 2003·Clinical & Experimental Metastasis·Brunhilde Felding-Habermann
Jul 29, 2003·Journal of Cancer Research and Clinical Oncology·Pia WülfingLudwig Kiesel
Nov 19, 2003·The Journal of Steroid Biochemistry and Molecular Biology·G L S Davies
Dec 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sung-Hee ChangTimothy Hla
Jan 22, 2004·British Journal of Cancer·G P BolandN J Bundred
Mar 17, 2004·The Journal of Biological Chemistry·K M Faisal KhanDomenick J Falcone
Mar 17, 2004·The Journal of Cell Biology·Hui WangRuth J Muschel
Jan 1, 2005·The Journal of Membrane Biology·Tsutomu Tsuji
Jul 29, 2005·Nature·Andy J MinnJoan Massagué
Mar 1, 2006·The Journal of Biological Chemistry·Gary K Yiu, Alex Toker
Mar 31, 2006·Molecular Biology of the Cell·Nicole E WinterwoodChristopher S Stipp
May 27, 2006·Endothelium : Journal of Endothelial Cell Research·Gian Carlo Alghisi, Curzio Rüegg
Apr 26, 2007·Nature Reviews. Cancer·M Peter Marinkovich
May 2, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Roger Stupp, Curzio Ruegg
May 26, 2007·The American Journal of Pathology·Jenny ChiaNormand Pouliot
Jul 13, 2007·Breast Cancer Research and Treatment·Gurpreet Singh-RangerKefah Mokbel
Sep 4, 2007·Cancer Metastasis Reviews·Man-Tzu WangDaotai Nie
Oct 15, 2008·The Journal of Biological Chemistry·Gouri BaldwinFedor Berditchevski
Mar 18, 2009·PloS One·Kushagra BansalKithiganahalli Narayanaswamy Balaji
Jun 18, 2009·Molecular Cancer Research : MCR·Rafal SadejFedor Berditchevski

❮ Previous
Next ❯

Citations

Apr 19, 2011·Breast Cancer Research and Treatment·Dong-Mei Li, Yu-Mei Feng
Apr 17, 2012·International Journal of Cell Biology·David G Menter, Raymond N Dubois
Nov 26, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Peeyush N Goel, Rajiv P Gude
Sep 6, 2013·Molecular Biology of the Cell·Jenny G ParvaniWilliam P Schiemann
Apr 16, 2014·Cancer radiothérapie : journal de la Société française de radiothérapie oncologique·K I Cao, Y M Kirova
Aug 16, 2011·Expert Opinion on Therapeutic Targets·Sita Subbaram, C Michael Dipersio
Jan 1, 2015·International Journal of Molecular Sciences·Feng-Chi Chen
Jun 20, 2015·Cytokine & Growth Factor Reviews·Ka Kui ChanMohamed El-Tanani
May 5, 2017·Cancer Growth and Metastasis·Satya S SadhuXiangming Guan
Aug 29, 2018·British Journal of Pharmacology·Debashree BasudharDavid A Wink
Oct 14, 2017·F1000Research·Whitney Longmate, C Michael DiPersio
Jul 7, 2011·Journal of Cellular Biochemistry·Indrajyoti Indra, Karen A Beningo
Nov 1, 2013·Oncology Reports·Sujitra DetchokulAlbert G Frauman
Jul 11, 2014·Anti-cancer Drugs·Cui-Cui SunZu-Hua Gao
Mar 10, 2011·International Journal of Cancer. Journal International Du Cancer·Nicole KusumaNormand Pouliot
Oct 27, 2012·Breast Cancer Research : BCR·Brigitte BisaroPaola Defilippi
Aug 11, 2016·Journal of Cellular Biochemistry·Lipsa DasAnne E Cress
May 30, 2019·Cancers·C Michael DiPersio, Livingston Van De Water
Jan 5, 2019·Journal of Cell Science·Jiahui Cao, Hans Schnittler
May 19, 2019·Breast Cancer Research : BCR·Veronika RamovsArnoud Sonnenberg
May 20, 2020·Life Science Alliance·Veronika RamovsArnoud Sonnenberg
Apr 19, 2017·The Journal of Cell Biology·Whitney M LongmateC Michael DiPersio
Apr 6, 2017·Frontiers in Pharmacology·Nadeem BilaniWassim Abou-Kheir
Jul 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Oh Yun KwonSeung Ho Lee
Nov 5, 2016·Cancer Research·Afshin VarzavandChristopher S Stipp
May 27, 2020·The Journal of Investigative Dermatology·Whitney M LongmateC Michael DiPersio
Nov 30, 2019·Neoplasia : an International Journal for Oncology Research·Hongxia LiXiuwei H Yang
Feb 17, 2019·Cancer Letters·Romain LévêqueRobert-Alain Toillon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Tumorigenesis

Breast tumorigenesis involves the production or formation of tumor(s) in breast tissue. Discover the latest research on breast tumorigenesis here.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.