PMID: 16525643Mar 10, 2006Paper

Suppression of invasive characteristics by antisense introduction of overexpressed HOX genes in ovarian cancer cells

International Journal of Oncology
Tsuyoshi YamashitaMutsuo Ishikawa

Abstract

HOX genes encode transcription factors that function to establish basic body pattern during embryogenesis and maintain the function of specific organs in the adult. Recent studies have demonstrated that HOX genes are also involved in oncogenesis in a range of malignancies. To elucidate whether HOX genes contribute to ovarian carcinogenesis, we created an expression profile of HOX genes using ovarian derived materials from surgical samples and epithelial ovarian cancer cells derived from five different cell lines. Real-time quantitative RT-PCR assay indicated overexpression of 14 HOX genes in clusters A and B but only 2 genes in clusters C and D. Of the 16 HOX genes, overexpression of paralogs of HOX3, HOX4 and HOX7 is seen in cluster A and B, and of HOX13 in all paralogs. In addition, HOXB7, HOXA13 and HOXB13 showed high levels of overexpression in cancer cells and tissues whereas no or little expression was observed in normal controls. To examine whether overexpressed HOX genes regulate invasion of ovarian cancer cells directly, we introduced an antisense DNA fragment of overexpressed HOXB7 and HOXB13, and HOXC5 that did not show overexpression into SKOV3 cells by electroporation. Antisense introduction followed by chemoinvasi...Continue Reading

Citations

Jul 6, 2014·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Seema BhatlekarBruce M Boman
May 19, 2010·Cellular and Molecular Life Sciences : CMLS·Simone BraigAnja-Katrin Bosserhoff
Apr 13, 2013·Journal de gynécologie, obstétrique et biologie de la reproduction·B BorgheseC Chapron
Apr 20, 2010·Journal de gynécologie, obstétrique et biologie de la reproduction·B BorgheseC Chapron
Mar 14, 2009·Differentiation; Research in Biological Diversity·Takayo OtaNelly Auersperg
Sep 18, 2009·The Journal of Investigative Dermatology·Judith A Mack, Edward V Maytin
Oct 24, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·A P G CrijnsE G E de Vries
Mar 10, 2016·Gynecologic Oncology·Katherine R MillerRam N Ganapathi
Sep 13, 2011·Journal of Ovarian Research·Zoë L KellyRichard Gl Morgan
Nov 18, 2009·Molecular Cell·John D NorrisDonald P McDonnell
May 27, 2016·International Journal of Cancer. Journal International Du Cancer·Zoe KellyAgnieszka Michael
Sep 30, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Xiao-Jun MaMark G Erlander

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