Suppression of lymphocyte apoptosis in spleen by CXCL13 after porcine circovirus type 2 infection and regulatory mechanism of CXCL13 expression in pigs

Veterinary Research
Gen LiuYunliang Jiang

Abstract

Porcine circovirus-associated disease (PCVAD) is one of the most serious infectious diseases in pigs worldwide. The primary causative agent of PCVAD is porcine circovirus type 2 (PCV2), which can cause lymphoid depletion and immunosuppression in pigs. Our previous study demonstrated that Laiwu (LW) pigs, a Chinese indigenous pig breed, have stronger resistance to PCV2 infection than Yorkshire × Landrace (YL) pigs. In this study, we found that the YL pigs showed more severe lymphocyte apoptosis and higher viral load in the spleen tissue than LW pigs. To illustrate the differential gene expression between healthy and infected spleens, transcriptome profiling of spleen tissues from PCV2-infected and control YL pigs was compared by RNA sequencing. A total of 90 differentially expressed genes (DEGs) was identified, including CD207, RSAD2, OAS1, OAS2, MX2, ADRB3, CXCL13, CCR1, and ADRA2C, which were significantly enriched in gene ontology (GO) terms related to the defense response to virus and cell-cell signaling, and another nine DEGs, KLF11, HGF, PTGES3, MAP3K11, XDH, CYCS, ACTC1, HSPH1, and RYR2, which were enriched in GO terms related to regulation of cell proliferation or apoptosis. Among these DEGs, the CXCL13 gene, which can s...Continue Reading

References

Feb 26, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·G M Allan, J A Ellis
Dec 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·K ShiT Ochi
Dec 29, 2000·The Journal of Veterinary Medical Science·T ShibaharaK Kadota
Mar 29, 2001·Nature Immunology·B Moser, P Loetscher
Sep 13, 2003·Immunological Reviews·Gerd MüllerMartin Lipp
Oct 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tomoya KatakaiAkira Shimizu
Dec 19, 2003·Nucleic Acids Research·Minoru KanehisaMasahiro Hattori
Dec 6, 2005·Journal of Comparative Pathology·T OpriessnigP G Halbur
Nov 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hu ChunsongTan Jinquan
Aug 29, 2007·Immunity·Christopher D C AllenJason G Cyster
Nov 14, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Tanja OpriessnigPatrick G Halbur
Feb 12, 2008·Seminars in Immunology·Christopher D C Allen, Jason G Cyster
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
May 1, 2012·Research in Veterinary Science·Yingjun LvShuxia Zhang
Apr 27, 2013·Journal of Molecular Signaling·Chris Yk Cheung, Ben Cb Ko
Jan 1, 2013·Annual Review of Animal Biosciences·Xiang-Jin Meng
Oct 23, 2015·Journal of Clinical and Experimental Hematopathology : JCEH·Haruo OhtaniNaoya Nakamura
Mar 21, 2019·Animal Models and Experimental Medicine·Ting OuyangLin-Zhu Ren

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Methods Mentioned

BETA
light microscopy
Fluorescence
PCR
cDNA library construction
RNA-seq
transfection
Assay
genotyping
flow cytometry

Software Mentioned

SPSS
Cufflinks
DESeq
Mx3000p
RegRNA
DAVID
DNAMAN
Tophat
KEGG
MatInspector

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis