Suppression of methanogenesis in hydrogen fermentation by intermittent feeding

Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
Jeonghee YunKyung-Suk Cho

Abstract

This study investigated whether intermittent feeding by using a concentrated carbon source is an appropriate method for selective enrichment of hydrogenesis by means of methanogen suppression. In a conventional reactor fed continuously for 10 d, methanogens increased from 2.8 × 10(7) to 1.1 × 10(9) gene copy number (GCN)/mg-cell dry weight, and methane concentration in the resulting biogas was 5.8%. However, when a carbon source was intermittently supplied for 10 d to the reactor, the number of methanogens was reduced 98.9% from 2.77 × 10(7) to 1.2 × 10(3) GCN/mg-cell dry weight, and methane was not detected during this period of intermittent feeding. Intermittent feeding shifted the dominants in the reactor from Clostridiaceae (70.5%) and Lactobacillaceae (11.0%) to Acetobacteraceae (62.0%) and Clostridiaceae (38.0%). In the reactor operated in continuous feeding mode after intermittent feeding, methane concentration was below 0.3% and the portion of methanogens in the bacterial community was maintained below 0.2%. These results suggest that the intermittent feeding of a carbon source during hydrogen production processes is a suitable method to suppress the activity of methanogens.

References

Aug 22, 2002·Water Research·Wen-Tso LiuHerbert H P Fang
Mar 29, 2006·Water Environment Research : a Research Publication of the Water Environment Federation·Samir Kumar KhanalShihwu Sung
Jun 24, 2008·Critical Reviews in Biotechnology·Peter Raspor, Dusan Goranovic
Jan 4, 2012·Applied Biochemistry and Biotechnology·Eduardo Lucena Cavalcante de AmorimEdson Luiz Silva
May 11, 2012·Applied Microbiology and Biotechnology·Tae Gwan KimKyung-Suk Cho
Oct 17, 2014·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Luis A López-EscobarJuan M Méndez-Contreras

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Methods Mentioned

BETA
chemical treatment
PCR
electrophoresis

Software Mentioned

Mothur

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