Suppression of microRNA-135b-5p protects against myocardial ischemia/reperfusion injury by activating JAK2/STAT3 signaling pathway in mice during sevoflurane anesthesia.

Bioscience Reports
Xiao-Juan XieLi-Gang Ma

Abstract

The study aims to explore the effects of miR-135b-5p on myocardial ischemia/reperfusion (I/R) injuries by regulating Janus protein tyrosine kinase 2 (JAK2)/signal transducer and activator of transcription (STAT) signaling pathway by mediating inhalation anesthesia with sevoflurane. A sum of 120 healthy Wistar male mice was assigned into six groups. Left ventricular ejection fraction (LVEF) and left ventricular shortening fraction (LVSF) were detected. Cardiomyocyte apoptosis was determined by terminal dexynucleotidyl transferase mediated dUTP-biotin nick end labeling (TUNEL) assay. MiR-135b-5p expression, mRNA and protein expression of p-STAT3, p-JAK2, STAT3, JAK2, B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein B (Bax) were detected by quantitative real-time PCR (qRT-PCR) and Western blotting. Target relationship between miR-135b-5p and JAK2 was confirmed by dual-luciferase reporter assay. The other five groups exhibited increased cardiomyocyte necrosis, apoptosis, miR-135b-5p and Bax expression, mRNA expression of JAK2 and STAT3, and protein expression of p-STAT3 and p-JAK2 compared with the sham group, but showed decreased LVEF, LVFS, and Bcl-2 expression. Compared with the model and AG490 + Sevo groups, the Sevo, i...Continue Reading

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Citations

Aug 22, 2020·Cancer Biotherapy & Radiopharmaceuticals·Yufeng GaoDongnan Hou
Apr 23, 2020·The Kaohsiung Journal of Medical Sciences·Xiang-Tao ZhangFei Wang
Oct 9, 2020·Immunological Investigations·Fatemeh Sabet Sarvestani, Negar Azarpira
Sep 9, 2020·Journal of the Chinese Medical Association : JCMA·Mengxia ZhongDalong Wang
May 18, 2020·International Immunopharmacology·Gholamreza Rezaei DanbaranHamed Mohammadi
Dec 31, 2020·International Journal of Molecular Sciences·Stephen M NiemiecCarlos Zgheib
Jun 27, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wu LiuZeYao Tang
Jul 23, 2021·Human & Experimental Toxicology·Bo SongHong-Yan Sun

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Methods Mentioned

BETA
PCR
coronary artery block

Software Mentioned

SPSS

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