Suppression of murine mammary carcinoma growth and metastasis by HSVtk/GCV gene therapy using in vivo electroporation

Cancer Gene Therapy
Masa-Aki ShibataYoshinori Otsuki

Abstract

The effectiveness of electroporation as a means of gene transfection, both in vitro and in vivo, was tested using the herpes simplex virus 1 thymidine kinase (HSVtk) gene in combination with ganciclovir (GCV) administration as therapy against murine mammary cancer. Approximately 80% of BJMC3879 metastatic mammary carcinoma cells, derived from MMTV-infected BALB/c mice, died as a result of HSVtk/GCV treatment 72 hours after the transfection; decreased DNA synthesis was also seen. Mammary tumors induced by inoculation of syngeneic mice with BJMC3879 cells were subsequently treated by direct injection of vector containing HSVtk (pHSVtk) alone, empty vector or saline alone twice a week. After each injection, the tumors were subjected to in vivo electroporation. Mice treated with pHSVtk or saline were intraperitoneally injected with GCV at 40 mg/kg five times a week. Significantly reduced tumor volumes were observed for the pHSVtk+GCV group in experimental week 2 and thereafter throughout the 2-month study. DNA synthesis was significantly decreased as well in the pHSVtk+GCV group compared with all other groups. Furthermore, metastasis to lymph nodes and lungs was significantly suppressed by HSVtk/GCV treatment. Expression of HSVtk i...Continue Reading

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Citations

May 14, 2005·Cancer Gene Therapy·Weitao HuangPaul Schwarzenberger
Sep 16, 2006·Human Gene Therapy·Loree C Heller, Richard Heller
Jul 14, 2010·The Journal of Membrane Biology·Jean-Michel EscoffreMarie-Pierre Rols
May 13, 2008·Medical Molecular Morphology·Masa-Aki ShibataYoshinori Otsuki
Dec 12, 2012·Medical Molecular Morphology·Masa-Aki ShibataMariko Harada-Shiba
Sep 21, 2007·Expert Opinion on Drug Delivery·Yoshitaka Isaka, Enyu Imai
Nov 22, 2005·Expert Opinion on Drug Delivery·Loree C HellerRichard Heller
Sep 1, 2003·Modern Rheumatology·Rie KatayamaRyuichi Gejo
Mar 11, 2003·Technology in Cancer Research & Treatment·Daniel SchermanMichel Francis Bureau
Oct 29, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Tomoaki GotoYukitaka Ushio
Feb 23, 2018·Cancer Immunology, Immunotherapy : CII·Urska KamensekGregor Sersa
Sep 21, 2006·Molecular Cancer Therapeutics·Hiroki MatsubaraTsuneharu Miki
Jul 19, 2014·Cancer Research·Sabina SangalettiClaudia Chiodoni

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