Suppression of myocardial protein degradation in the rat during fasting. Effects of insulin, glucose, and leucine

Circulation Research
G D CurfmanT W Smith

Abstract

To study the effects of leucine, glucose, and insulin on myocardial protein degradation in fed and fasted nutritional states, we developed and validated a sensitive method for measuring rates of total protein degradation in rat isolated left atrial preparations. Fasting resulted in a progressive decrease in myocardial protein breakdown to 71% of control over a 24-hour period, with no further reduction in degradation rate between 24 and 72 hours of fasting. Insulin (100 mU/ml) suppressed atrial protein degradation by 38% in fed animals (P less than 0.001) and by 51% in fasted animals (P less than 0.001). Glucose alone had no effect on protein degradation in either nutritional state. At 5 times normal plasma levels, leucine suppressed protein breakdown by 21% in fed and by 15% in fasted animals. The decrease in degradation induced by fasting and the absence of an effect of glucose are in contrast to the behavior reported for skeletal muscle.

References

Nov 1, 1975·Biulleten' eksperimental'noĭ biologii i meditsiny·I P Gereliuk, S M Mints
Jan 1, 1976·Annual Review of Biochemistry·A L Goldberg, A C St John
May 15, 1977·The Biochemical Journal·M F HopgoodF J Ballard
Aug 1, 1976·The American Journal of Physiology·J B Li, A L Goldberg
Nov 1, 1975·The Journal of Clinical Investigation·M G Buse, S S Reid
Jan 1, 1974·Annual Review of Biochemistry·A L Goldberg, J F Dice
Dec 1, 1972·The American Journal of Physiology·R Odessey, A L Goldberg
Oct 1, 1972·The Biochemical Journal·L KelmanJ Terblanche
Mar 1, 1974·The American Journal of Physiology·D E RannelsH E Morgan
Mar 19, 1970·The New England Journal of Medicine·G F Cahill
Jan 1, 1969·The American Journal of Physiology·J CohenA A Whitbeck
Apr 1, 1967·The American Journal of Physiology·J R NeelyH E Morgan

❮ Previous
Next ❯

Citations

Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J P ReevesK Wildenthal
Aug 5, 1981·Biochimica Et Biophysica Acta·B Grinde, P O Seglen
Jan 1, 1989·Life Sciences·J M Fagan, M E Tischler
Mar 1, 1989·Diabetes/metabolism Reviews·E J Barrett, R A Gelfand
Aug 1, 1986·The Journal of Surgical Research·C WittnichR C Chiu
Jul 1, 1981·Experimental Cell Research·B Grinde, P O Seglen
Jun 1, 1986·The American Journal of Physiology·A F ClarkK Wildenthal
Nov 1, 1989·The American Journal of Physiology·N A SchroedlC R Hartzell
Sep 1, 1984·The American Journal of Physiology·M E TischlerJ Coffman
Aug 1, 1982·Archives of Biochemistry and Biophysics·M E Tischler, J M Fagan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.