Suppression of natural killer cells in the presence of CD34+ blood progenitor cells and peripheral blood lymphocytes

Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation
J ClausenE Gunsilius

Abstract

Abstract Mobilization of CD34 + peripheral blood progenitor cells (PBPCs) with granulocyte-colony stimulating factor (G-CSF) may induce functional alterations in peripheral blood lymphocyte (PBL) subsets. We and others have shown that natural killer (NK) cells from PBPC collections are less expandable in vitro than those obtained during steady-state hematopoiesis. We show here that the extent of this proliferation deficit is related to the number of circulating CD34 + cells in vivo at the time of PBPC apheresis. Likewise, addition of autologous CD34 + cells to unseparated PBL reduced the expansion of the NK-cell subset by 22.2% +/- 6.0% (n = 10; P <.005). In contrast, when using purified NK cells, their proliferation remained unimpaired by autologous CD34 + cells. Supernatants from CD34 + cells cultured with autologous PBLs had an inhibitory effect on proliferation of purified NK cells (n = 16; P =.03), indicating that an interaction between CD34 + cells and lymphocytes is essential for the suppressive effect on NK cells. To investigate the role of T cells in this interaction, intracellular cytokines were determined in T cells cultured for 7 days with or without autologous CD34 + cells. When cultured with CD34 + cells, the freq...Continue Reading

References

Nov 4, 1996·International Journal of Cancer. Journal International Du Cancer·H RabinowichT L Whiteside
Apr 1, 1997·British Journal of Haematology·D BehringerA Lindemann

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Citations

Oct 28, 2009·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Johannes ClausenDavid Nachbaur
Nov 5, 2010·Clinical and Vaccine Immunology : CVI·Luigi BuonaguroFranco M Buonaguro
Sep 27, 2018·International Journal of Molecular Sciences·Tor Henrik Anderson TvedtØystein Bruserud

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